Universität zu Köln

Busch, Anna and Hess, Sebastian (2017). The Cytoskeleton Architecture of Algivorous Protoplast Feeders (Viridiraptoridae, Rhizaria) Indicates Actin-Guided Perforation of Prey Cell Walls. Protist, 168 (1). S. 12 - 32. JENA: ELSEVIER GMBH, URBAN & FISCHER VERLAG. ISSN 1434-4610

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Abstract

Several distantly related, phagotrophic microeukaryotes share the remarkable ability to perforate foreign cell walls in a well-defined pattern to acquire protoplast material as food. The underlying cellular processes, especially the local application of cell wall degrading agents, are still unexplored. We examined the distribution of F-actin and alpha-tubulin in the algivorous, viridiraptorid amoeboflagellates Orciraptor agilis and Viridiraptor invadens over their life histories using phalloidin conjugates and immunolocalization. During attack, both species form distinctive, F-actin-rich structures at the contact zone to the algal prey cell, which exactly match the species-specific cell wall perforations and resemble invadopodia and podosome rosettes of mammalian cells to a certain extent. Furthermore, F-actin is involved in the extraction of plastid material by Orciraptorand in prey cell invasion by Viridiraptor(here, F-actin localizes to a characteristic hyaline channel, which surrounds the streaming cytoplasm). The digestive -reproductive stages of viridiraptorids display a highly ordered microtubular cytoskeleton, whereas distinct phalloidin-positive actin structures could not be detected. We discuss the presumed function of F-actin during perforation and penetration of the algal cell wall by viridiraptorids, and compare the cytoskeleton architecture of these protoplast feeders to amoeboflagellates from different eukaryotic supergroups. (C)2016 Elsevier GmbH. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Busch, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Hess, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-240617
DOI:	10.1016/j.protis.2016.10.004
Journal or Publication Title:	Protist
Volume:	168
Number:	1
Page Range:	S. 12 - 32
Date:	2017
Publisher:	ELSEVIER GMBH, URBAN & FISCHER VERLAG
Place of Publication:	JENA
ISSN:	1434-4610
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ROOT HAIR-CELLS; F-ACTIN; PHYSARUM-POLYCEPHALUM; MYOSIN-II; FLUORESCENCE MICROSCOPY; PARAMECIUM-TETRAURELIA; POLYCLONAL ANTIBODIES; COCHLIOBOLUS-SATIVUS; FLAGELLAR APPARATUS; BREVIATA-ANATHEMA Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/24061