Universität zu Köln

Hillebrand, Frank, Peter, Jan Otto, Brillen, Anna-Lena, Otte, Marianne ORCID: 0000-0001-7433-2444, Schaal, Heiner and Erkelenz, Steffen (2017). Differential hnRNP D isoform incorporation may confer plasticity to the ESSV-mediated repressive state across HIV-1 exon 3. Biochim. Biophys. Acta-Gene Regul. Mech., 1860 (2). S. 205 - 218. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 0006-3002

Full text not available from this repository.

Abstract

Even though splicing repression by hnRNP complexes bound to exonic sequences is well-documented, the responsible effector domains of hnRNP proteins have been described for only a select number of hnRNP constituents. Thus, there is only limited information available for possible varying silencer activities amongst different hnRNP proteins and composition changes within possible hnRNP complex assemblies. In this study, we identified the glycine-rich domain (GRD) of hnRNP proteins as a unifying feature in splice site repression. We also show that all four hnRNP D isoforms can act as genuine splicing repressors when bound to exonic positions. The presence of an extended GRD, however, seemed to potentiate the hnRNP D silencer activity of isoforms p42 and p45. Moreover, we demonstrate that hnRNP D proteins associate with the HIV-1 ESSV silencer complex, probably through direct recognition of UUAG sequences overlapping with the previously described UAGG motifs bound by hnRNP A1. Consequently, this spatial proximity seems to cause mutual interference between hnRNP A1 and hnRNP D. This interplay between hnRNP A1 and D facilitates a dynamic regulation of the repressive state of HIV-1 exon 3 which manifests as fluctuating relative levels of spliced vpr- and unspliced gag/pol-mRNAs. (C) 2016 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hillebrand, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Peter, Jan Otto UNSPECIFIED UNSPECIFIED UNSPECIFIED Brillen, Anna-Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Otte, Marianne UNSPECIFIED orcid.org/0000-0001-7433-2444 UNSPECIFIED Schaal, Heiner UNSPECIFIED UNSPECIFIED UNSPECIFIED Erkelenz, Steffen UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-240899
DOI:	10.1016/j.bbagrm.2016.12.001
Journal or Publication Title:	Biochim. Biophys. Acta-Gene Regul. Mech.
Volume:	1860
Number:	2
Page Range:	S. 205 - 218
Date:	2017
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	0006-3002
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IMMUNODEFICIENCY-VIRUS TYPE-1; PRE-MESSENGER-RNA; TRACT-BINDING-PROTEIN; INTRONIC G RUN; SPLICING SILENCER; TAT EXON-2; GENE-EXPRESSION; A/B PROTEINS; REPLICATION; SITE Multiple languages Biochemistry & Molecular Biology; Biophysics Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/24089