Universität zu Köln

Arlt, Christian, Flegler, Vanessa, Ihling, Christian H., Schaefer, Mathias ORCID: 0000-0002-5943-4335, Thondorf, Iris and Sinz, Andrea (2017). An Integrated Mass Spectrometry Based Approach to Probe the Structure of the Full-Length Wild-Type Tetrameric p53 Tumor Suppressor. Angew. Chem.-Int. Edit., 56 (1). S. 275 - 280. WEINHEIM: WILEY-V C H VERLAG GMBH. ISSN 1521-3773

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Abstract

We present an integrated approach for investigating the topology of proteins through native mass spectrometry (MS) and cross-linking/MS, which we applied to the full-length wild-type p53 tetramer. For the first time, the two techniques were combined in one workflow to obtain not only structural insight in the p53 tetramer, but also information on the cross-linking efficiency and the impact of cross-linker modification on the conformation of an intrinsically disordered protein (IDP). P53 cross-linking was monitored by native MS and as such, our strategy serves as a quality control for different cross-linking reagents. Our approach can be applied to the structural investigation of various protein systems, including IDPs and large protein assemblies, which are challenging to study by the conventional methods used for protein structure characterization.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Arlt, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Flegler, Vanessa UNSPECIFIED UNSPECIFIED UNSPECIFIED Ihling, Christian H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaefer, Mathias UNSPECIFIED orcid.org/0000-0002-5943-4335 UNSPECIFIED Thondorf, Iris UNSPECIFIED UNSPECIFIED UNSPECIFIED Sinz, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-242835
DOI:	10.1002/anie.201609826
Journal or Publication Title:	Angew. Chem.-Int. Edit.
Volume:	56
Number:	1
Page Range:	S. 275 - 280
Date:	2017
Publisher:	WILEY-V C H VERLAG GMBH
Place of Publication:	WEINHEIM
ISSN:	1521-3773
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Organic Chemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CHEMICAL CROSS-LINKING; PROTEIN-INTERACTION; DNA RECOGNITION; COMPLEX; BINDING; CONFORMATIONS; BIOLOGY; DOMAIN; MS Multiple languages Chemistry, Multidisciplinary Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/24283