Universität zu Köln

Carpenter, Philip M., Sivadas, Priyanka, Hua, Spencer S., Xiao, Cally ORCID: 0000-0001-7172-3175, Gutierrez, Alyssa B., Ngo, Tuan and Gershon, Paul D. (2017). Migration of breast cancer cell lines in response to pulmonary laminin 332. Cancer Med., 6 (1). S. 220 - 235. HOBOKEN: WILEY. ISSN 2045-7634

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Abstract

Because tumor cell motility is a requirement for metastasis, we hypothesized that lung tissue harbors substances that induce tumor cell migration. MCF-7 breast carcinoma cells exposed to small airway epithelial cells and conditioned medium exhibited dose-dependent tumor cell migration. Among the extracellular matrix proteins in the conditioned medium identified by mass spectrometry, laminin 332 (LM332) had the greatest contribution to the migration of MCF-7 cells. Immunoblotting and immunohistochemistry for LM332-specific chains identified LM332 in the lung and in pulmonary epithelial cells. Antibodies to either LM332 or its integrin receptor inhibited MCF-7 motility, and knockdown of LM332 chains also reduced its migration-inducing activity. Taken together, these findings implicate LM332 as a component of lung tissue that can induce motility in breast carcinoma cells that have been transported to lung during metastasis. Earlier studies on LM332 in tumor progression have examined LM332 expression in tumor cells. This investigation, in comparison, provides evidence that the tumor promoting potential of LM332 may originate in the lung microenvironment rather than in tumor cells alone. Furthermore, this study provides evidence that the motility-inducing properties of the microenvironment can reside in epithelial cells. The findings raise the possibility that LM332 plays a role in the pulmonary metastases of breast carcinoma and may provide a target for antimetastasis therapy.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Carpenter, Philip M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sivadas, Priyanka UNSPECIFIED UNSPECIFIED UNSPECIFIED Hua, Spencer S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Xiao, Cally UNSPECIFIED orcid.org/0000-0001-7172-3175 UNSPECIFIED Gutierrez, Alyssa B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ngo, Tuan UNSPECIFIED UNSPECIFIED UNSPECIFIED Gershon, Paul D. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-247352
DOI:	10.1002/cam4.957
Journal or Publication Title:	Cancer Med.
Volume:	6
Number:	1
Page Range:	S. 220 - 235
Date:	2017
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	2045-7634
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	CECAD - Cluster of Excellence Cellular Stress Responses in Aging-Associated Diseases
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXTRACELLULAR-MATRIX; TUMOR INVASION; ALPHA-3-BETA-1 INTEGRIN; EPITHELIAL-CELLS; INDUCED MOTILITY; ADHESION; CARCINOMA; MARKER; KINASE; GROWTH Multiple languages Oncology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/24735