Universität zu Köln

Chacon-Martinez, Carlos Andres, Klose, Markus, Niemann, Catherin, Glauche, Ingmar and Wickstroem, Sara A. (2017). Hair follicle stem cell cultures reveal self-organizing plasticity of stem cells and theirprogeny. Embo J., 36 (2). S. 151 - 165. HOBOKEN: WILEY-BLACKWELL. ISSN 1460-2075

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Abstract

Understanding how complex tissues are formed, maintained, and regenerated through local growth, differentiation, and remodeling requires knowledge on how single-cell behaviors are coordinated on the population level. The self-renewing hair follicle, maintained by a distinct stem cell population, represents an excellent paradigm to address this question. A major obstacle in mechanistic understanding of hair follicle stem cell (HFSC) regulation has been the lack of a culture system that recapitulates HFSC behavior while allowing their precise monitoring and manipulation. Here, we establish an invitro culture system based on a 3D extracellular matrix environment and defined soluble factors, which for the first time allows expansion and long-term maintenance of murine multipotent HFSCs in the absence of heterologous cell types. Strikingly, this scheme promotes de novo generation of HFSCs from non-HFSCs and vice versa in a dynamic self-organizing process. This bidirectional interconversion of HFSCs and their progeny drives the system into a population equilibrium state. Our study uncovers regulatory dynamics by which phenotypic plasticity of cells drives population-level homeostasis within a niche, and provides a discovery tool for studies on adult stem cell fate.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Chacon-Martinez, Carlos Andres UNSPECIFIED UNSPECIFIED UNSPECIFIED Klose, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Niemann, Catherin UNSPECIFIED UNSPECIFIED UNSPECIFIED Glauche, Ingmar UNSPECIFIED UNSPECIFIED UNSPECIFIED Wickstroem, Sara A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-247555
DOI:	10.15252/embj.201694902
Journal or Publication Title:	Embo J.
Volume:	36
Number:	2
Page Range:	S. 151 - 165
Date:	2017
Publisher:	WILEY-BLACKWELL
Place of Publication:	HOBOKEN
ISSN:	1460-2075
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENDOTHELIAL GROWTH-FACTOR; EPITHELIAL-CELLS; NICHE; REGENERATION; DYNAMICS; DIFFERENTIATION; EXPRESSION; ENRICHMENT; MECHANISM; LINEAGE Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/24755