Universität zu Köln

Thomas, Andreas ORCID: 0000-0003-1199-0743, Brinkkoetter, Paul T., Schaenzer, Wilhelm and Thevis, Mario (2017). Simultaneous determination of insulin, DesB30 insulin, proinsulin, and C-peptide in human plasma samples by liquid chromatography coupled to high resolution mass spectrometry. Forensic Toxicol., 35 (1). S. 106 - 114. NEW YORK: SPRINGER. ISSN 1860-8973

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Abstract

The endocrine pancreatic processes comprising the main actions of insulin, proinsulin, and C-peptide, represent a well-investigated system in the human organism because of the widespread epidemic of diabetes mellitus. Besides their medical relevance, insulin and its related compounds have further been of particular interest also to forensic science and doping controls, and in all disciplines, a specific and sensitive determination of the target analytes is critical for accurate result interpretation. In the present study, a method was developed allowing for the simultaneous determination of human insulin (and its synthetic analogues), the degradation product DesB30 human insulin, proinsulin, and C-peptide in plasma samples (100 A mu L) by liquid chromatography coupled to high resolution mass spectrometry. Immunoaffinity extraction employing magnetic nanoparticles and two monoclonal antibodies for insulin and C-peptide were used in combination with two internal standards. The assay was validated for quantitative result evaluation considering the parameters specificity, linearity (0-10 ng/mL), limit of detection (similar to 0.05 ng/mL), precision at high and low levels (6-25%), accuracy at high and low levels (85-127%), recovery (33-44%), ion suppression, and stability. Furthermore, the method was applied to samples from healthy athletes, patients with diabetes mellitus (type II) with and without treatment with insulin, and patients being treated for diabetes mellitus (type I). The results enable a thorough interpretation of the data with respect to endocrine, forensic and doping control samples.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Thomas, Andreas UNSPECIFIED orcid.org/0000-0003-1199-0743 UNSPECIFIED Brinkkoetter, Paul T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaenzer, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-247992
DOI:	10.1007/s11419-016-0343-8
Journal or Publication Title:	Forensic Toxicol.
Volume:	35
Number:	1
Page Range:	S. 106 - 114
Date:	2017
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1860-8973
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DOPING CONTROL PURPOSES; ISOTOPE-DILUTION ASSAY; INTACT INSULIN; LC-MS/MS; IMMUNOAFFINITY PURIFICATION; SYNTHETIC ANALOGS; QUANTIFICATION; SERUM; IMMUNOASSAYS; QUANTITATION Multiple languages Toxicology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/24799