Universität zu Köln

Bock, Felix, Onderka, Jasmine, Braun, Gabriele, Schneider, Ann-Charlott, Hos, Deniz, Bi, Yanlong, Bachmann, Bjoern O. and Cursiefen, Claus (2016). Identification of Novel Endogenous Anti(lymph)angiogenic Factors in the Aqueous Humor. Invest. Ophthalmol. Vis. Sci., 57 (15). S. 6554 - 6561. ROCKVILLE: ASSOC RESEARCH VISION OPHTHALMOLOGY INC. ISSN 1552-5783

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Abstract

PURPOSE. The avascular cornea is in direct contact with aqueous humor (AqH). Here we investigate whether AqH exerts anti(lymph) angiogenic effects and thereby may contribute to corneal (lymph) angiogenic privilege. METHODS. Using the murine model of suture-induced inflammatory corneal hem-and lymphangiogenesis, the potential anti(lymph) angiogenic effect of AqH was analyzed by applying murine AqH as eyedrops. Anti(lymph) angiogenic effects were measured using morphometric analysis of flat mounts stained with CD31 as panendothelial and LYVE-1 as specific lymphatic endothelial marker. The potential antilymphangiogenic effect of immunomodulatory factors contained in AqH such as vasoactive intestinal peptide (VIP) and alpha-melanocyte stimulating hormone (alpha-MSH) was analyzed in lymphatic and blood vascular endothelial cell proliferation assays in vitro. RESULTS. Topically applied AqH significantly inhibited corneal hemangiogenesis and even more so lymphangiogenesis in vivo and directly in vitro. The immunoregulatory factors VIP and alpha-MSH significantly inhibited lymphatic endothelial cell proliferation in vitro. Depletion of VIP or alpha-MSH from AqH diminished its anti-hem-and lymphangiogenic potential. CONCLUSIONS. Aqueous humor exerts significant antilymphangiogenic effects in vivo. This is at least partially mediated by the known immunomodulatory factors VIP and alpha-MSH present in the AqH. Therefore, AqH not only contributes to corneal lymphangiogenic privilege and is a new tool to identify novel endogenous regulators of lymphangiogenesis but also may have therapeutic applications.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Bock, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED Onderka, Jasmine UNSPECIFIED UNSPECIFIED UNSPECIFIED Braun, Gabriele UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Ann-Charlott UNSPECIFIED UNSPECIFIED UNSPECIFIED Hos, Deniz UNSPECIFIED UNSPECIFIED UNSPECIFIED Bi, Yanlong UNSPECIFIED UNSPECIFIED UNSPECIFIED Bachmann, Bjoern O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cursiefen, Claus UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-253647
DOI:	10.1167/iovs.15-18526
Journal or Publication Title:	Invest. Ophthalmol. Vis. Sci.
Volume:	57
Number:	15
Page Range:	S. 6554 - 6561
Date:	2016
Publisher:	ASSOC RESEARCH VISION OPHTHALMOLOGY INC
Place of Publication:	ROCKVILLE
ISSN:	1552-5783
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MELANOCYTE-STIMULATING HORMONE; GROWTH-FACTOR-BETA; IMMUNOSUPPRESSIVE FACTOR; CARCINOMA-CELLS; CORNEAL; LYMPHANGIOGENESIS; NEOVASCULARIZATION; HEMANGIOGENESIS; ANGIOGENESIS; INHIBITION Multiple languages Ophthalmology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/25364