Universität zu Köln

Roempler, Katharina, Mueller, Tobias, Juris, Lisa, Wissel, Mirjam, Vukotic, Milena, Hofmann, Kay ORCID: 0000-0002-2289-9083 and Deckers, Markus (2016). Overlapping Role of Respiratory Supercomplex Factor Rcf2 and Its N-terminal Homolog Rcf3 in Saccharomyces cerevisiae. J. Biol. Chem., 291 (45). S. 23769 - 23779. BETHESDA: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC. ISSN 1083-351X

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Abstract

The mitochondrial electron transport chain consists of individual protein complexes arranged into large macromolecular structures, termed respiratory chain supercomplexes or respirasomes. In the yeast Saccharomyces cerevisiae, respiratory chain supercomplexes form by association of the bc(1) complex with the cytochrome c oxidase. Formation and maintenance of these assemblies are promoted by specific respiratory supercomplex factors, the Rcf proteins. For these proteins a regulatory function in bridging the electron transfer within supercomplexes has been proposed. Here we report on the maturation of Rcf2 into an N- and C-terminal peptide. We show that the previously uncharacterized Rcf3 (YBR255c-A) is a homolog of the N-terminal Rcf2 peptide, whereas Rcf1 is homologous to the C-terminal portion. Both Rcf3 and the C-terminal fragment of Rcf2 associate with monomeric cytochrome c oxidase and respiratory chain supercomplexes. A lack of Rcf2 and Rcf3 increases oxygen flux through the respiratory chain by up-regulation of the cytochrome c oxidase activity. A double gene deletion of RCF2 and RCF3 affects cellular survival under non-fermentable growth conditions, suggesting an overlapping role for both proteins in the regulation of the OXPHOS activity. Furthermore, our data suggest an association of all three Rcf proteins with the bc(1) complex in the absence of a functional cytochrome c oxidase and identify a supercomplex independent interaction network of the Rcf proteins.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Roempler, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Juris, Lisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Wissel, Mirjam UNSPECIFIED UNSPECIFIED UNSPECIFIED Vukotic, Milena UNSPECIFIED UNSPECIFIED UNSPECIFIED Hofmann, Kay UNSPECIFIED orcid.org/0000-0002-2289-9083 UNSPECIFIED Deckers, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-255869
DOI:	10.1074/jbc.M116.734665
Journal or Publication Title:	J. Biol. Chem.
Volume:	291
Number:	45
Page Range:	S. 23769 - 23779
Date:	2016
Publisher:	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Place of Publication:	BETHESDA
ISSN:	1083-351X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CYTOCHROME-C-OXIDASE; MITOCHONDRIAL-MEMBRANE PROTEIN; OXIDATIVE-PHOSPHORYLATION; CHAIN SUPERCOMPLEXES; YEAST; COMPLEX; ORGANIZATION; CARDIOLIPIN; BIOGENESIS; COMPONENTS Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/25586