Universität zu Köln

Ghatak, Sushmita, Niland, Stephan, Schulz, Jan-Niklas ORCID: 0000-0001-9286-206X, Wang, Fang, Eble, Johannes A., Leitges, Michael, Mauch, Cornelia, Krieg, Thomas, Zigrino, Paola ORCID: 0000-0002-7470-0064 and Eckes, Beate (2016). Role of Integrins alpha 1 beta 1 and alpha 2 beta 1 in Wound and Tumor Angiogenesis in Mice. Am. J. Pathol., 186 (11). S. 3011 - 3028. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1525-2191

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Abstract

Integrins are transmembrane receptors composed of one alpha subunit and one beta subunit and are involved in cellular growth, differentiation, and apoptosis. The collagen-binding integrins alpha 1 beta 1 and alpha 2 beta 1 have been shown to regulate wound and tumor vascularization by different mechanisms. In this study, we assessed wound and tumor vascularization in mice with genetic ablation of both integrin subunits alpha 1 and alpha 2, which resulted in Loss of integrins alpha 1 beta 1 and ant Wound angiogenesis was investigated in excisional wounds that were inflicted on the back skin of control and mice lacking integrin alpha 1 beta 1 and alpha 2 beta 1. Mutant mice displayed reduced wound angiogenesis, which correlated with decreased macrophage numbers at 3 and 7 days after injury, and showed significantly attenuated vascularization of sponge implants. Angiogenesis induced by tumors arising from intradermal injection of B16 F1 melanoma cells was also reduced in comparison to controls 7 days after injection. This reduction in angiogenesis correlated with increased Levels and activity of circulating matrix metalloproteinase 9 and elevated angiostatin levels in plasma of mutant mice, which reduced endothelial cell proliferation. Ex vivo mutant aortic ring explants developed significantly fewer and thinner aortic sprouts with fewer branch points than controls because of impaired endothelial cell proliferation. In conclusion, the loss of integrins alpha 1 beta 1 and alpha 2 beta 1 in mice results in reduced wound and tumor angiogenesis by cell-autonomous and extrinsic mechanisms.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ghatak, Sushmita UNSPECIFIED UNSPECIFIED UNSPECIFIED Niland, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Schulz, Jan-Niklas UNSPECIFIED orcid.org/0000-0001-9286-206X UNSPECIFIED Wang, Fang UNSPECIFIED UNSPECIFIED UNSPECIFIED Eble, Johannes A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Leitges, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Mauch, Cornelia UNSPECIFIED UNSPECIFIED UNSPECIFIED Krieg, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Zigrino, Paola UNSPECIFIED orcid.org/0000-0002-7470-0064 UNSPECIFIED Eckes, Beate UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-257219
DOI:	10.1016/j.ajpath.2016.06.021
Journal or Publication Title:	Am. J. Pathol.
Volume:	186
Number:	11
Page Range:	S. 3011 - 3028
Date:	2016
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1525-2191
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language COLLAGEN-BINDING INTEGRINS; CELL-CYCLE PROGRESSION; IN-VITRO; ALPHA(2)BETA(1) INTEGRINS; GRANULATION-TISSUE; EXTRACELLULAR-MATRIX; MURINE DEVELOPMENT; ALPHA-1-NULL MICE; BASEMENT-MEMBRANE; RECOGNITION SITE Multiple languages Pathology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/25721