Asaoka, Tomoko ORCID: 0000-0001-5141-2353, Almagro, Jorge, Ehrhardt, Christine, Tsai, Isabella, Schleiffer, Alexander ORCID: 0000-0001-6251-2747, Deszcz, Luiza, Junttila, Sini, Ringrose, Leonie, Mechtler, Karl ORCID: 0000-0002-3392-9946, Kavirayani, Anoop, Gyenesei, Attila, Hofmann, Kay ORCID: 0000-0002-2289-9083, Duchek, Peter, Rittinger, Katrin ORCID: 0000-0002-7698-4435 and Ikeda, Fumiyo ORCID: 0000-0003-0407-2768 (2016). Linear ubiquitination by LUBEL has a role in Drosophila heat stress response. EMBO Rep., 17 (11). S. 1624 - 1641. HOBOKEN: WILEY. ISSN 1469-3178

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Abstract

The HOIP ubiquitin E3 ligase generates linear ubiquitin chains by forming a complex with HOIL-1L and SHARPIN in mammals. Here, we provide the first evidence of linear ubiquitination induced by a HOIP orthologue in Drosophila. We identify Drosophila CG11321, which we named Linear Ubiquitin E3 ligase (LUBEL), and find that it catalyzes linear ubiquitination in vitro. We detect endogenous linear ubiquitin chain-derived peptides by mass spectrometry in Drosophila Schneider 2 cells and adult flies. Furthermore, using CRISPR/Cas9 technology, we establish linear ubiquitination-defective flies by mutating residues essential for the catalytic activity of LUBEL. Linear ubiquitination signals accumulate upon heat shock in flies. Interestingly, flies with LUBEL mutations display reduced survival and climbing defects upon heat shock, which is also observed upon specific LUBEL depletion in muscle. Thus, LUBEL is involved in the heat response by controlling linear ubiquitination in flies.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Asaoka, TomokoUNSPECIFIEDorcid.org/0000-0001-5141-2353UNSPECIFIED
Almagro, JorgeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ehrhardt, ChristineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tsai, IsabellaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schleiffer, AlexanderUNSPECIFIEDorcid.org/0000-0001-6251-2747UNSPECIFIED
Deszcz, LuizaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Junttila, SiniUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ringrose, LeonieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mechtler, KarlUNSPECIFIEDorcid.org/0000-0002-3392-9946UNSPECIFIED
Kavirayani, AnoopUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gyenesei, AttilaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hofmann, KayUNSPECIFIEDorcid.org/0000-0002-2289-9083UNSPECIFIED
Duchek, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rittinger, KatrinUNSPECIFIEDorcid.org/0000-0002-7698-4435UNSPECIFIED
Ikeda, FumiyoUNSPECIFIEDorcid.org/0000-0003-0407-2768UNSPECIFIED
URN: urn:nbn:de:hbz:38-257709
DOI: 10.15252/embr.201642378
Journal or Publication Title: EMBO Rep.
Volume: 17
Number: 11
Page Range: S. 1624 - 1641
Date: 2016
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1469-3178
Language: English
Faculty: Faculty of Mathematics and Natural Sciences
Divisions: Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
NF-KAPPA-B; CHAIN ASSEMBLY COMPLEX; INTEGRATIVE GENOMICS VIEWER; ENHANCER FACTOR-2 MEF2; ZINC-FINGER 7; POLYUBIQUITIN CHAINS; CELL-DEATH; SIGNALING COMPLEX; BINDING ENTITIES; GENE-EXPRESSIONMultiple languages
Biochemistry & Molecular Biology; Cell BiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/25770

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