Universität zu Köln

Klein, Brianna J., Wang, Xiaoyan, Cui, Gaofeng, Yuan, Chao, Botuyan, Maria Victoria, Lin, Kevin, Lu, Yue ORCID: 0000-0002-5479-9095, Wang, Xiaolu, Zhao, Yue, Bruns, Christiane J., Mer, Georges, Shi, Xiaobing ORCID: 0000-0001-5242-8189 and Kutateladze, Tatiana G. (2016). PHF20 Readers Link Methylation of Histone H3K4 and p53 with H4K16 Acetylation. Cell Reports, 17 (4). S. 1158 - 1171. CAMBRIDGE: CELL PRESS. ISSN 2211-1247

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Abstract

PHF20 is a core component of the lysine acetyltransferase complex MOF (male absent on the first)-NSL (non-specific lethal) that generates the major epigenetic mark H4K16ac and is necessary for transcriptional regulation and DNA repair. The role of PHF20 in the complex remains elusive. Here, we report on functional coupling between methylation readers in PHF20. We show that the plant homeodomain (PHD) finger of PHF20 recognizes dimethylated lysine 4 of histone H3 (H3K4me2) and represents an example of a native reader that selects for this modification. Biochemical and structural analyses help to explain this selectivity and the preference of Tudor2, another reader in PHF20, for dimethylated p53. Binding of the PHD finger to H3K4me2 is required for histone acetylation, accumulation of PHF20 at target genes, and transcriptional activation. Together, our findings establish a unique PHF20-mediated link between MOF histone acetyltransferase (HAT), p53, and H3K4me2, and suggest a model for rapid spreading of H4K16ac-enriched open chromatin.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Klein, Brianna J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Xiaoyan UNSPECIFIED UNSPECIFIED UNSPECIFIED Cui, Gaofeng UNSPECIFIED UNSPECIFIED UNSPECIFIED Yuan, Chao UNSPECIFIED UNSPECIFIED UNSPECIFIED Botuyan, Maria Victoria UNSPECIFIED UNSPECIFIED UNSPECIFIED Lin, Kevin UNSPECIFIED UNSPECIFIED UNSPECIFIED Lu, Yue UNSPECIFIED orcid.org/0000-0002-5479-9095 UNSPECIFIED Wang, Xiaolu UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhao, Yue UNSPECIFIED UNSPECIFIED UNSPECIFIED Bruns, Christiane J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mer, Georges UNSPECIFIED UNSPECIFIED UNSPECIFIED Shi, Xiaobing UNSPECIFIED orcid.org/0000-0001-5242-8189 UNSPECIFIED Kutateladze, Tatiana G. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-258320
DOI:	10.1016/j.celrep.2016.09.056
Journal or Publication Title:	Cell Reports
Volume:	17
Number:	4
Page Range:	S. 1158 - 1171
Date:	2016
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2211-1247
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GLIOMA-EXPRESSED ANTIGEN-2; MOLECULAR RECOGNITION; LYSINE METHYLATION; CHROMATIN-BINDING; EFFECTOR PROTEIN; STRUCTURAL BASIS; CHEMICAL-SHIFT; PHD FINGER; MODEL; MOF Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/25832