Jung, Sang-Eun, Cho, Sohee, Antunes, Joana ORCID: 0000-0003-3614-3233, Gomes, Iva ORCID: 0000-0002-1593-301X, Uchimoto, Mari L., Oh, Yu Na, Di Giacomo, Lisa, Schneider, Peter M. ORCID: 0000-0003-0744-2349, Park, Min Sun, van der Meer, Dieudonne ORCID: 0000-0001-9913-7872, Williams, Graham, McCord, Bruce, Ahn, Hee-Jung, Choi, Dong Ho, Lee, Yang Han, Lee, Soong Deok and Lee, Hwan Young (2016). A collaborative exercise on DNA methylation based body fluid typing. Electrophoresis, 37 (21). S. 2759 - 2767. HOBOKEN: WILEY-BLACKWELL. ISSN 1522-2683

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Abstract

A collaborative exercise on DNA methylation based body fluid identification was conducted by seven laboratories. For this project, a multiplex methylation SNaPshot reaction composed of seven CpG markers was used for the identification of four body fluids, including blood, saliva, semen, and vaginal fluid. A total of 30 specimens were prepared and distributed to participating laboratories after thorough testing. The required experiments included four increasingly complex tasks: (1) CE of a purified single-base extension reaction product, (2) multiplex PCR and multiplex single-base extension reaction of bisulfite-modified DNA, (3) bisulfite conversion of genomic DNA, and (4) extraction of genomic DNA from body fluid samples. In tasks 2, 3 and 4, one or more mixtures were analyzed, and specimens containing both known and unknown body fluid sources were used. Six of the laboratories generated consistent body fluid typing results for specimens of bisulfite-converted DNA and genomic DNA. One laboratory failed to set up appropriate conditions for capillary analysis of reference single-base extension products. In general, variation in the values obtained for DNA methylation analysis between laboratories increased with the complexity of the required experiments. However, all laboratories concurred on the interpretation of the DNA methylation profiles produced. Although the establishment of interpretational guidelines on DNA methylation based body fluid identification has yet to be performed, this study supports the addition of DNA methylation profiling to forensic body fluid typing.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Jung, Sang-EunUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cho, SoheeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Antunes, JoanaUNSPECIFIEDorcid.org/0000-0003-3614-3233UNSPECIFIED
Gomes, IvaUNSPECIFIEDorcid.org/0000-0002-1593-301XUNSPECIFIED
Uchimoto, Mari L.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Oh, Yu NaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Di Giacomo, LisaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schneider, Peter M.UNSPECIFIEDorcid.org/0000-0003-0744-2349UNSPECIFIED
Park, Min SunUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
van der Meer, DieudonneUNSPECIFIEDorcid.org/0000-0001-9913-7872UNSPECIFIED
Williams, GrahamUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
McCord, BruceUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ahn, Hee-JungUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Choi, Dong HoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lee, Yang HanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lee, Soong DeokUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lee, Hwan YoungUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-261454
DOI: 10.1002/elps.201600256
Journal or Publication Title: Electrophoresis
Volume: 37
Number: 21
Page Range: S. 2759 - 2767
Date: 2016
Publisher: WILEY-BLACKWELL
Place of Publication: HOBOKEN
ISSN: 1522-2683
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
RNA/DNA CO-ANALYSIS; EDNAP EXERCISE; MENSTRUAL BLOOD; MESSENGER-RNA; IDENTIFICATION; MARKERS; STAINS; SALIVA; SEMENMultiple languages
Biochemical Research Methods; Chemistry, AnalyticalMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/26145

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