Universität zu Köln

Cosgaya, Clara, Mari-Almirall, Marta, Van Assche, Ado ORCID: 0000-0002-5016-0489, Fernandez-Orth, Dietmar, Mosqueda, Noraida, Telli, Murat, Huys, Geert ORCID: 0000-0002-7092-4837, Higgins, Paul G. ORCID: 0000-0001-8677-9454, Seifert, Harald, Lievens, Bart, Roca, Ignasi ORCID: 0000-0002-1800-1576 and Vila, Jordi ORCID: 0000-0002-8025-3926 (2016). Acinetobacter dijkshoorniae sp nov., a member of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex mainly recovered from clinical samples in different countries. Int. J. Syst. Evol. Microbiol., 66. S. 4105 - 4112. LONDON: MICROBIOLOGY SOC. ISSN 1466-5034

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Abstract

The recent advances in bacterial species identification methods have led to the rapid taxonomic diversification of the genus Acinetobacter. In the present study, phenotypic and molecular methods have been used to determine the taxonomic position of a group of 12 genotypically distinct strains belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex, initially described by Gerner-Smidt and Tjernberg in 1993, that are closely related to Acinetobacter pittii. Strains characterized in this study originated mostly from human samples obtained in different countries over a period of 15 years. rpoB gene sequences and multilocus sequence typing were used for comparisons against 94 strains representing all species included in the ACB complex. Cluster analysis based on such sequences showed that all 12 strains grouped together in a distinct clade closest to Acinetobacter pittiithat was supported by bootstrap values of 99 %. Values of average nucleotide identity based on BLAST between the genome sequence of strain JVAP01(T) (NCBI accession no. LJPG00000000) and those of other species from the ACB complex were always <91.2 %, supporting the species status of the group. In addition, the metabolic characteristics of the group matched those of the ACB complex and the analysis of their protein signatures by matrix-assisted laser desorption ionization time-of-flight MS identified some specific peaks. Our results support the designation of these strains as representing a novel species, for which the name Acinetobacter dijkshoorniae sp. nov. is proposed. The type strain is JVAP01(T) (= CECT 9134(T) = LMG 29605(T)).

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Cosgaya, Clara UNSPECIFIED UNSPECIFIED UNSPECIFIED Mari-Almirall, Marta UNSPECIFIED UNSPECIFIED UNSPECIFIED Van Assche, Ado UNSPECIFIED orcid.org/0000-0002-5016-0489 UNSPECIFIED Fernandez-Orth, Dietmar UNSPECIFIED UNSPECIFIED UNSPECIFIED Mosqueda, Noraida UNSPECIFIED UNSPECIFIED UNSPECIFIED Telli, Murat UNSPECIFIED UNSPECIFIED UNSPECIFIED Huys, Geert UNSPECIFIED orcid.org/0000-0002-7092-4837 UNSPECIFIED Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Lievens, Bart UNSPECIFIED UNSPECIFIED UNSPECIFIED Roca, Ignasi UNSPECIFIED orcid.org/0000-0002-1800-1576 UNSPECIFIED Vila, Jordi UNSPECIFIED orcid.org/0000-0002-8025-3926 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-261567
DOI:	10.1099/ijsem.0.001318
Journal or Publication Title:	Int. J. Syst. Evol. Microbiol.
Volume:	66
Page Range:	S. 4105 - 4112
Date:	2016
Publisher:	MICROBIOLOGY SOC
Place of Publication:	LONDON
ISSN:	1466-5034
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NATURAL SOIL; RPOB GENE; IDENTIFICATION; PITTII Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/26156