Universität zu Köln

Flegel, Caroline, Vogel, Felix, Hofreuter, Adrian, Wojcik, Sebastian, Schoeder, Clara, Kiec-Kononowicz, Katarzyna ORCID: 0000-0002-6752-7443, Brockmeyer, Norbert H., Mueller, Christa E., Becker, Christian, Altmueller, Janine, Hatt, Hanns and Gisselmann, Gunter (2016). Characterization of non-olfactory GPCRs in human sperm with a focus on GPR18. Sci Rep, 6. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

Full text not available from this repository.

Abstract

G protein-coupled receptors (GPCRs) transduce external chemical cues into intracellular signals and are involved in a plethora of physiological processes, but knowledge regarding the function of these receptors in spermatozoa is limited. In the present study, we performed RNA-Seq and analyzed the expression of the all GPCRs except olfactory receptors in human spermatozoa. We revealed the expression of up to 223 different GPCR transcripts in human spermatozoa (FPKM > 0.1) and identified GPR18, a newly described cannabinoid receptor, together with GPR137 and GPR135, as one of the three most highly expressed GPCRs. To date, the expression of GPR18 was completely unknown in human spermatozoa. We confirmed GPR18 expression using RT-PCR and immuncytochemistry experiments and localized the GPR18 protein in the midpiece of human spermatozoa. Stimulation of human spermatozoa with the GPR18 ligand N-arachidonoylglycine induced the phosphorylation of 12 protein kinases, some of them are for example known to be involved in the acrosome reaction. In line with this, N-arachidonoylglycine affected the cytoskeleton by changing levels of F-actin and inducing the acrosome reaction in human spermatozoa in a concentration-dependent manner. Our results indicate that GPR18 might be involved in physiological processes of human spermatozoa, suggesting GPR18 to be a potential player in sperm physiology.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Flegel, Caroline UNSPECIFIED UNSPECIFIED UNSPECIFIED Vogel, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED Hofreuter, Adrian UNSPECIFIED UNSPECIFIED UNSPECIFIED Wojcik, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Schoeder, Clara UNSPECIFIED UNSPECIFIED UNSPECIFIED Kiec-Kononowicz, Katarzyna UNSPECIFIED orcid.org/0000-0002-6752-7443 UNSPECIFIED Brockmeyer, Norbert H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Christa E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Altmueller, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED Hatt, Hanns UNSPECIFIED UNSPECIFIED UNSPECIFIED Gisselmann, Gunter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-266197
DOI:	10.1038/srep32255
Journal or Publication Title:	Sci Rep
Volume:	6
Date:	2016
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ACTIN CYTOSKELETON; HUMAN SPERMATOZOA; N-ARACHIDONYLGLYCINE; ACROSOME REACTION; PHOSPHOLIPASE-C; ZONA-PELLUCIDA; CAPACITATION; IDENTIFICATION; EXPRESSION; RECEPTORS Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/26619