Universität zu Köln

Corman, Victor M., Eckerle, Isabella, Memish, Ziad A., Liljander, Anne M., Dijkman, Ronald ORCID: 0000-0003-0320-2743, Jonsdottir, Hulda, Ngeiywa, Kisi J. Z. Juma, Kamau, Esther, Younan, Mario, Al Masri, Malakita, Assiri, Abdullah ORCID: 0000-0002-5605-2876, Gluecks, Ilona, Musa, Bakri E., Meyer, Benjamin ORCID: 0000-0003-0601-3550, Mueller, Marcel A., Hilali, Mosaad, Bornstein, Set, Wernery, Ulrich, Thiel, Volker ORCID: 0000-0002-5783-0887, Jores, Joerg ORCID: 0000-0003-3790-5746, Drexler, Jan Felix ORCID: 0000-0002-3509-0232 and Drosten, Christian (2016). Link of a ubiquitous human coronavirus to dromedary camels. Proc. Natl. Acad. Sci. U. S. A., 113 (35). S. 9864 - 9870. WASHINGTON: NATL ACAD SCIENCES. ISSN 0027-8424

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Abstract

The four human coronaviruses (HCoVs) are globally endemic respiratory pathogens. The Middle East respiratory syndrome (MERS) coronavirus (CoV) is an emerging CoV with a known zoonotic source in dromedary camels. Little is known about the origins of endemic HCoVs. Studying these viruses' evolutionary history could provide important insight into CoV emergence. In tests of MERS-CoV-infected dromedaries, we found viruses related to an HCoV, known as HCoV-229E, in 5.6% of 1,033 animals. Human-and dromedary-derived viruses are each monophyletic, suggesting ecological isolation. One gene of dromedary viruses exists in two versions in camels, full length and deleted, whereas only the deleted version exists in humans. The deletion increased in size over a succession starting from camelid viruses via old human viruses to contemporary human viruses. Live isolates of dromedary 229E viruses were obtained and studied to assess human infection risks. The viruses used the human entry receptor aminopeptidase N and replicated in human hepatoma cells, suggesting a principal ability to cause human infections. However, inefficient replication in several mucosa-derived cell lines and airway epithelial cultures suggested lack of adaptation to the human host. Dromedary viruses were as sensitive to the human type I interferon response as HCoV-229E. Antibodies in human sera neutralized dromedary-derived viruses, suggesting population immunity against dromedary viruses. Although no current epidemic risk seems to emanate from these viruses, evolutionary inference suggests that the endemic human virus HCoV-229E may constitute a descendant of camelid-associated viruses. HCoV-229E evolution provides a scenario for MERS-CoV emergence.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Corman, Victor M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Eckerle, Isabella UNSPECIFIED UNSPECIFIED UNSPECIFIED Memish, Ziad A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Liljander, Anne M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dijkman, Ronald UNSPECIFIED orcid.org/0000-0003-0320-2743 UNSPECIFIED Jonsdottir, Hulda UNSPECIFIED UNSPECIFIED UNSPECIFIED Ngeiywa, Kisi J. Z. Juma UNSPECIFIED UNSPECIFIED UNSPECIFIED Kamau, Esther UNSPECIFIED UNSPECIFIED UNSPECIFIED Younan, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED Al Masri, Malakita UNSPECIFIED UNSPECIFIED UNSPECIFIED Assiri, Abdullah UNSPECIFIED orcid.org/0000-0002-5605-2876 UNSPECIFIED Gluecks, Ilona UNSPECIFIED UNSPECIFIED UNSPECIFIED Musa, Bakri E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyer, Benjamin UNSPECIFIED orcid.org/0000-0003-0601-3550 UNSPECIFIED Mueller, Marcel A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hilali, Mosaad UNSPECIFIED UNSPECIFIED UNSPECIFIED Bornstein, Set UNSPECIFIED UNSPECIFIED UNSPECIFIED Wernery, Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Thiel, Volker UNSPECIFIED orcid.org/0000-0002-5783-0887 UNSPECIFIED Jores, Joerg UNSPECIFIED orcid.org/0000-0003-3790-5746 UNSPECIFIED Drexler, Jan Felix UNSPECIFIED orcid.org/0000-0002-3509-0232 UNSPECIFIED Drosten, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-266208
DOI:	10.1073/pnas.1604472113
Journal or Publication Title:	Proc. Natl. Acad. Sci. U. S. A.
Volume:	113
Number:	35
Page Range:	S. 9864 - 9870
Date:	2016
Publisher:	NATL ACAD SCIENCES
Place of Publication:	WASHINGTON
ISSN:	0027-8424
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RESPIRATORY SYNDROME CORONAVIRUS; RECEPTOR-BINDING DOMAIN; MERS CORONAVIRUS; SAUDI-ARABIA; AMINOPEPTIDASE-N; 229E RECEPTOR; IDENTIFICATION; VIRUS; ANTIBODIES; SPIKE Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/26620