Universität zu Köln

Chan, Kai Xun ORCID: 0000-0003-3554-7228, Mabbitt, Peter D., Phua, Su Yin ORCID: 0000-0002-7556-1211, Mueller, Jonathan W., Nisar, Nazia, Gigolashvili, Tamara ORCID: 0000-0002-0416-4796, Stroeher, Elke, Grassl, Julia ORCID: 0000-0001-8653-4983, Arlt, Wiebke ORCID: 0000-0001-5106-9719, Estavillo, Gonzalo M., Jackson, Colin J. and Pogson, Barry J. (2016). Sensing and signaling of oxidative stress in chloroplasts by inactivation of the SAL1 phosphoadenosine phosphatase. Proc. Natl. Acad. Sci. U. S. A., 113 (31). S. E4567 - 10. WASHINGTON: NATL ACAD SCIENCES. ISSN 0027-8424

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Abstract

Intracellular signaling during oxidative stress is complex, with organelle-to-nucleus retrograde communication pathways ill-defined or incomplete. Here we identify the 3'-phosphoadenosine 5'-phosphate (PAP) phosphatase SAL1 as a previously unidentified and conserved oxidative stress sensor in plant chloroplasts. Arabidopsis thaliana SAL1 (AtSAL1) senses changes in photosynthetic redox poise, hydrogen peroxide, and superoxide concentrations in chloroplasts via redox regulatory mechanisms. AtSAL1 phosphatase activity is suppressed by dimerization, intramolecular disulfide formation, and glutathionylation, allowing accumulation of its substrate, PAP, a chloroplast stress retrograde signal that regulates expression of plastid redox associated nuclear genes (PRANGs). This redox regulation of SAL1 for activation of chloroplast signaling is conserved in the plant kingdom, and the plant protein has evolved enhanced redox sensitivity compared with its yeast ortholog. Our results indicate that in addition to sulfur metabolism, SAL1 orthologs have evolved secondary functions in oxidative stress sensing in the plant kingdom.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Chan, Kai Xun UNSPECIFIED orcid.org/0000-0003-3554-7228 UNSPECIFIED Mabbitt, Peter D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Phua, Su Yin UNSPECIFIED orcid.org/0000-0002-7556-1211 UNSPECIFIED Mueller, Jonathan W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Nisar, Nazia UNSPECIFIED UNSPECIFIED UNSPECIFIED Gigolashvili, Tamara UNSPECIFIED orcid.org/0000-0002-0416-4796 UNSPECIFIED Stroeher, Elke UNSPECIFIED UNSPECIFIED UNSPECIFIED Grassl, Julia UNSPECIFIED orcid.org/0000-0001-8653-4983 UNSPECIFIED Arlt, Wiebke UNSPECIFIED orcid.org/0000-0001-5106-9719 UNSPECIFIED Estavillo, Gonzalo M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Jackson, Colin J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Pogson, Barry J. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-266842
DOI:	10.1073/pnas.1604936113
Journal or Publication Title:	Proc. Natl. Acad. Sci. U. S. A.
Volume:	113
Number:	31
Page Range:	S. E4567 - 10
Date:	2016
Publisher:	NATL ACAD SCIENCES
Place of Publication:	WASHINGTON
ISSN:	0027-8424
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NORMAL-MODE ANALYSIS; REDOX REGULATION; ALLOSTERIC INHIBITION; SINGLET OXYGEN; ARABIDOPSIS; PROTEIN; DROUGHT; GLUTATHIONE; EXPRESSION; MECHANISM Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/26684