Universität zu Köln

Nickel, Nadine ORCID: 0000-0002-0609-0735, Cleven, Astrid, Enders, Vitalij, Lisak, Dmitrij, Schneider, Lars and Methner, Axel ORCID: 0000-0002-8774-0057 (2016). Androgen-inducible gene 1 increases the ER Ca2+ content and cell death susceptibility against oxidative stress. Gene, 586 (1). S. 62 - 69. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1879-0038

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Abstract

Androgen-induced gene 1 (AIG1) is a transmembrane protein implicated with survival (its expression level was shown to correlate with the survival of patients suffering from hepatocellular carcinoma) and Ca2+ signaling (over-expression of AIG1 increased transcription mediated by the Ca2+-dependent nuclear factor of activated T cells). We aimed to shed light on this less-studied protein and investigated its tissue expression, genomic organization, intracellular localization and membrane topology as well as its effects on cell death susceptibility and the Ca2+ content of the endoplasmic reticulum. Immunoblotting of mouse tissues demonstrated highest expression of AIG1 in the liver, lung and heart. AIG1 has a complex genomic organization and expresses several splice variants in a tissue-dependent manner. Analyzing the topology of AIG1 in the ER membrane using a protease protection assay suggested that AIG has five transmembrane domains with a luminal N- and cytosolic C-terminus and a hydrophobic stretch between the third and fourth membrane domain that does not cross the membrane. AIG1 over-expression slightly increased susceptibility to oxidative stress, which correlated with an increased ER Ca2+ concentration in two different cell lines. Together, these results indicate that AIG1 plays a role in the control of the intracellular Ca2+ concentration and cell death susceptibility. (C) 2016 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Nickel, Nadine UNSPECIFIED orcid.org/0000-0002-0609-0735 UNSPECIFIED Cleven, Astrid UNSPECIFIED UNSPECIFIED UNSPECIFIED Enders, Vitalij UNSPECIFIED UNSPECIFIED UNSPECIFIED Lisak, Dmitrij UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Lars UNSPECIFIED UNSPECIFIED UNSPECIFIED Methner, Axel UNSPECIFIED orcid.org/0000-0002-8774-0057 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-269894
DOI:	10.1016/j.gene.2016.03.055
Journal or Publication Title:	Gene
Volume:	586
Number:	1
Page Range:	S. 62 - 69
Date:	2016
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	1879-0038
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BAX INHIBITOR-1; PROTEIN; REARRANGEMENTS Multiple languages Genetics & Heredity Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/26989