Universität zu Köln

Gschwendtner, Silvia, Mansfeldt, Tim ORCID: 0000-0002-7557-6827, Kublik, Susanne, Touliari, Evangelia, Buegger, Franz and Schloter, Michael (2016). Long-term ferrocyanide application via deicing salts promotes the establishment of Actinomycetales assimilating ferrocyanide-derived carbon in soil. Microb. Biotechnol., 9 (4). S. 502 - 514. HOBOKEN: WILEY-BLACKWELL. ISSN 1751-7915

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Abstract

Cyanides are highly toxic and produced by various microorganisms as defence strategy or to increase their competitiveness. As degradation is the most efficient way of detoxification, some microbes developed the capability to use cyanides as carbon and nitrogen source. However, it is not clear if this potential also helps to lower cyanide concentrations in roadside soils where deicing salt application leads to significant inputs of ferrocyanide. The question remains if biodegradation in soils can occur without previous photolysis. By conducting a microcosm experiment using soils with/without pre-exposition to road salts spiked with C-13-labelled ferrocyanide, we were able to confirm biodegradation and in parallel to identify bacteria using ferrocyanide as C source via DNA stable isotope probing (DNA-SIP), TRFLP fingerprinting and pyrosequencing. Bacteria assimilating C-13 were highly similar in the pre-exposed soils, belonging mostly to Actinomycetales (Kineosporia, Mycobacterium, Micromonosporaceae). In the soil without pre-exposition, bacteria belonging to Acidobacteria (Gp3, Gp4, Gp6), Gemmatimonadetes (Gemmatimonas) and Gammaproteobacteria (Thermomonas, Xanthomonadaceae) used ferrocyanide as C source but not the present Actinomycetales. This indicated that (i) various bacteria are able to assimilate ferrocyanide-derived C and (ii) long-term exposition to ferrocyanide applied with deicing salts leads to Actinomycetales outcompeting other microorganisms for the use of ferrocyanide as C source.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gschwendtner, Silvia UNSPECIFIED UNSPECIFIED UNSPECIFIED Mansfeldt, Tim UNSPECIFIED orcid.org/0000-0002-7557-6827 UNSPECIFIED Kublik, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Touliari, Evangelia UNSPECIFIED UNSPECIFIED UNSPECIFIED Buegger, Franz UNSPECIFIED UNSPECIFIED UNSPECIFIED Schloter, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-270859
DOI:	10.1111/1751-7915.12362
Journal or Publication Title:	Microb. Biotechnol.
Volume:	9
Number:	4
Page Range:	S. 502 - 514
Date:	2016
Publisher:	WILEY-BLACKWELL
Place of Publication:	HOBOKEN
ISSN:	1751-7915
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Geosciences > Geographisches Institut
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IRON-CYANIDE COMPLEXES; RIBOSOMAL-RNA; MICROBIAL BIOMASS; EXTRACTION METHOD; PURE CULTURES; ROAD SALT; DEGRADATION; RHIZOSPHERE; MICROORGANISMS; PSEUDOMONAS Multiple languages Biotechnology & Applied Microbiology; Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27085