Universität zu Köln

Mekonnen, Dereje Worku ORCID: 0000-0003-3167-7412 and Ludewig, Frank (2016). Phenotypic and chemotypic studies using Arabidopsis and yeast reveal that GHB converts to SSA and induce toxicity. Plant Mol.Biol., 91 (4-5). S. 429 - 441. DORDRECHT: SPRINGER. ISSN 1573-5028

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Abstract

gamma-Hydroxybutyric acid (GHB) is a naturally occurring compound. It is detected in organisms such as yeasts, plants and mammals. GHB is produced from the reduction of succinic semialdehyde (SSA) by the activity of GHB dehydrogenase. Arabidopsis genome contains two GHB dehydrogenase encoding genes. The accumulation of GHB in ssadh mutants led to the speculation that GHB is the cause of aberrant phenotypes. Conversely, the accumulation of GHB in Arabidopsis plants subjected to abiotic stresses was described as a way of avoiding SSA induced damage. To resolve these contrasting views on GHB, we examined the effect of exogenous GHB and SSA on the growth of yeast and Arabidopsis plants. GHB concentrations up to 1.5 mM didn't affect shoots of Arabidopsis plants; however, root growth was inhibited. In contrast, 0.3 mM SSA has severely affected the growth of plants. Treatment of yeast wild-type strain with 10 mM SSA and 10 mM GHB didn't affect the growth. However, the growth of yeast uga2 mutant was greatly inhibited by the same concentration of SSA, but not GHB. Metabolic analysis and enzyme activity assay on native gel showed that Arabidopsis, but not yeast, possesses a GHB dehydrogenase activity that converts GHB back to SSA. The enzymatic assay has also indicated the existence of an additional GHB dehydrogenase encoding gene(s) in Arabidopsis genome. Taken together, we conclude that GHB is less toxic than SSA. Its accumulation in ssadh mutants and during abiotic stresses is a response to avoid the SSA induced damage.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mekonnen, Dereje Worku UNSPECIFIED orcid.org/0000-0003-3167-7412 UNSPECIFIED Ludewig, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-270991
DOI:	10.1007/s11103-016-0475-6
Journal or Publication Title:	Plant Mol.Biol.
Volume:	91
Number:	4-5
Page Range:	S. 429 - 441
Date:	2016
Publisher:	SPRINGER
Place of Publication:	DORDRECHT
ISSN:	1573-5028
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GAMMA-HYDROXYBUTYRIC ACID; SUCCINATE SEMIALDEHYDE DEHYDROGENASE; SACCHAROMYCES-CEREVISIAE; GLYOXYLATE REDUCTASE; OXIDATIVE STRESS; ABIOTIC STRESS; EXPRESSION; THALIANA; MUTANTS; ACCUMULATION Multiple languages Biochemistry & Molecular Biology; Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27099