Universität zu Köln

Nuesken, Eva, Gellhaus, Alexandra, Kuehnel, Elisabeth, Swoboda, Isabelle, Wohlfarth, Maria, Vohlen, Christina, Schneider, Holm, Doetsch, Joerg and Nuesken, Kai-Dietrich (2016). Increased Rat Placental Fatty Acid, but Decreased Amino Acid and Glucose Transporters Potentially Modify Intrauterine Programming. J. Cell. Biochem., 117 (7). S. 1594 - 1604. HOBOKEN: WILEY. ISSN 1097-4644

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Abstract

Regulation of placental nutrient transport significantly affects fetal development and may modify intrauterine growth restriction (IUGR) and fetal programming. We hypothesized that placental nutrient transporters are differentially affected both by utero-placental insufficiency and prenatal surgical stress. Pregnant rats underwent bilateral uterine artery and vein ligation (LIG), sham operation (SOP) or no operation (controls, C) on gestational day E19. Placentas were obtained by caesarean section 4h (LIG, n=20 placentas; SOP, n=24; C, n=12), 24h (LIG, n=28; SOP, n=20; C, n=12) and 72h (LIG, n=20; SOP, n=20; C, n=24) after surgery. Gene and protein expression of placental nutrient transporters for fatty acids (h-FABP, CD36), amino acids (SNAT1, SNAT2) and glucose (GLUT-1, Connexin 26) were examined by qRT-PCR, western blot and immunohistochemistry. Interestingly, the mean protein expression of h-FABP was doubled in placentas of LIG and SOP animals 4, 24 (SOP significant) and 72h (SOP significant) after surgery. CD36 protein was significantly increased in LIG after 72h. SNAT1 and SNAT2 protein and gene expressions were significantly reduced in LIG and SOP after 24h. Further significantly reduced proteins were GLUT-1 in LIG (4h, 72h) and SOP (24h), and Connexin 26 in LIG (72h). In conclusion, placental nutrient transporters are differentially affected both by reduced blood flow and stress, probably modifying the already disturbed intrauterine milieu and contributing to IUGR and fetal programming. Increased fatty acid transport capacity may affect energy metabolism and could be a compensatory reaction with positive effects on brain development. (C) 2015 Wiley Periodicals, Inc.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Nuesken, Eva UNSPECIFIED UNSPECIFIED UNSPECIFIED Gellhaus, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuehnel, Elisabeth UNSPECIFIED UNSPECIFIED UNSPECIFIED Swoboda, Isabelle UNSPECIFIED UNSPECIFIED UNSPECIFIED Wohlfarth, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Vohlen, Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Holm UNSPECIFIED UNSPECIFIED UNSPECIFIED Doetsch, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED Nuesken, Kai-Dietrich UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-271562
DOI:	10.1002/jcb.25450
Journal or Publication Title:	J. Cell. Biochem.
Volume:	117
Number:	7
Page Range:	S. 1594 - 1604
Date:	2016
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1097-4644
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FETAL-GROWTH-RESTRICTION; EXPRESSION; PROTEIN; SYSTEM; MODEL; RETARDATION; RECEPTOR; BINDING; METABOLISM; PRESSURE Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27156