Universität zu Köln

Housley, Michael P., Njaine, Brian, Ricciardi, Filomena, Stone, Oliver A., Hoelper, Soraya, Krueger, Marcus ORCID: 0000-0003-2008-4582, Kostin, Sawa and Stainier, Didier Y. R. (2016). Cavin4b/Murcb Is Required for Skeletal Muscle Development and Function in Zebrafish. PLoS Genet., 12 (6). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1553-7404

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Abstract

Skeletal muscles provide metazoans with the ability to feed, reproduce and avoid predators. In humans, a heterogeneous group of genetic diseases, termed muscular dystrophies ( MD), lead to skeletal muscle dysfunction. Mutations in the gene encoding Caveolin-3, a principal component of the membrane micro-domains known as caveolae, cause defects in muscle maintenance and function; however it remains unclear how caveolae dysfunction underlies MD pathology. The Cavin family of caveolar proteins can form membrane remodeling oligomers and thus may also impact skeletal muscle function. Changes in the distribution and function of Cavin4/Murc, which is predominantly expressed in striated muscles, have been reported to alter caveolae structure through interaction with Caveolin-3. Here, we report the generation and phenotypic analysis of murcb mutant zebrafish, which display impaired swimming capacity, skeletal muscle fibrosis and T-tubule abnormalities during development. To understand the mechanistic importance of Murc loss of function, we assessed Caveolin-1 and 3 localization and found it to be abnormal. We further identified an in vivo function for Murc in Erk signaling. These data link Murc with developmental defects in T-tubule formation and progressive muscle dysfunction, thereby providing a new candidate for the etiology of muscular dystrophy.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Housley, Michael P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Njaine, Brian UNSPECIFIED UNSPECIFIED UNSPECIFIED Ricciardi, Filomena UNSPECIFIED UNSPECIFIED UNSPECIFIED Stone, Oliver A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoelper, Soraya UNSPECIFIED UNSPECIFIED UNSPECIFIED Krueger, Marcus UNSPECIFIED orcid.org/0000-0003-2008-4582 UNSPECIFIED Kostin, Sawa UNSPECIFIED UNSPECIFIED UNSPECIFIED Stainier, Didier Y. R. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-273057
DOI:	10.1371/journal.pgen.1006099
Journal or Publication Title:	PLoS Genet.
Volume:	12
Number:	6
Date:	2016
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1553-7404
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DUCHENNE MUSCULAR-DYSTROPHY; COILED-COIL PROTEIN; T-TUBULE BIOGENESIS; DILATED CARDIOMYOPATHY; GLYCOPROTEIN COMPLEX; EVANS BLUE; CAVEOLIN-3; MURC; SARCOLEMMA; ORGANIZATION Multiple languages Genetics & Heredity Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27305