Universität zu Köln

Shinde, Vaibhav, Brungs, Sonja, Hescheler, Juergen, Hemmersbach, Ruth and Sachinidis, Agapios (2016). Pipette-based Method to Study Embryoid Body Formation Derived from Mouse and Human Pluripotent Stem Cells Partially Recapitulating Early Embryonic Development Under Simulated Microgravity Conditions. Microgravity Sci. Technol., 28 (3). S. 287 - 296. NEW YORK: SPRINGER. ISSN 1875-0494

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Abstract

The in vitro differentiation of pluripotent stem cells partially recapitulates early in vivo embryonic development. More recently, embryonic development under the influence of microgravity has become a primary focus of space life sciences. In order to integrate the technique of pluripotent stem cell differentiation with simulated microgravity approaches, the 2-D clinostat compatible pipette-based method was experimentally investigated and adapted for investigating stem cell differentiation processes under simulated microgravity conditions. In order to keep residual accelerations as low as possible during clinorotation, while also guaranteeing enough material for further analysis, stem cells were exposed in 1-mL pipettes with a diameter of 3.5 mm. The differentiation of mouse and human pluripotent stem cells inside the pipettes resulted in the formation of embryoid bodies at normal gravity (1 g) after 24 h and 3 days. Differentiation of the mouse pluripotent stem cells on a 2-D pipette-clinostat for 3 days also resulted in the formation of embryoid bodies. Interestingly, the expression of myosin heavy chain was downregulated when cultivation was continued for an additional 7 days at normal gravity. This paper describes the techniques for culturing and differentiation of pluripotent stem cells and exposure to simulated microgravity during culturing or differentiation on a 2-D pipette clinostat. The implementation of these methodologies along with -omics technologies will contribute to understand the mechanisms regulating how microgravity influences early embryonic development.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Shinde, Vaibhav UNSPECIFIED UNSPECIFIED UNSPECIFIED Brungs, Sonja UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Hemmersbach, Ruth UNSPECIFIED UNSPECIFIED UNSPECIFIED Sachinidis, Agapios UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-273177
DOI:	10.1007/s12217-015-9469-2
Journal or Publication Title:	Microgravity Sci. Technol.
Volume:	28
Number:	3
Page Range:	S. 287 - 296
Date:	2016
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1875-0494
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DEVELOPMENT IN-VITRO; SKELETAL-MUSCLE; EXPRESSION; SPACEFLIGHT; TRANSCRIPTOMICS; CARDIOMYOCYTES; SIGNATURES; EXPOSURE Multiple languages Engineering, Aerospace; Thermodynamics; Mechanics Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27317