Universität zu Köln

Christjani, Mark, Fink, Patrick ORCID: 0000-0002-5927-8977 and von Elert, Eric ORCID: 0000-0001-7758-716X (2016). Phenotypic plasticity in three Daphnia genotypes in response to predator kairomone: evidence for an involvement of chitin deacetylases. J. Exp. Biol., 219 (11). S. 1697 - 1705. CAMBRIDGE: COMPANY BIOLOGISTS LTD. ISSN 1477-9145

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Abstract

The genetic background of inducible morphological defences in Daphnia is still largely unknown. Dissolved infochemicals from the aquatic larvae of the phantom midge Chaoborus induce so-called 'neck-teeth' in the first three post-embryonic stages of Daphnia pulex. This defence has becomea textbook example of inducible defences. In a target gene approach, by using three Daphnia genotypes which show a gradient of neck-teeth induction in response to equal amounts of kairomone, we report a high correlation of neck-teeth induction in Daphnia pulex and relative gene expression of two chitin deacetylases. Further, previous studies suggested genes from both the juvenoid and the insulin hormone signalling pathways as well as several morphogenetic genes downstream to be responsible for neck-teeth induction in D. pulex. However, these data were not supported by our study. None of the three D. pulex clones showed an upregulation of these previously proposed candidate genes as a response to predator kairomone, which is interpreted as the result of refined methods used for both RNA sampling and kairomone enrichment yielding unambiguous results compared with earlier studies. The assessment of a clonal gradient of Daphnia in the presence and absence of infochemicals provides a promising approach to identify further genes involved in the induction of morphological defences by correlating gene expression and morphology.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Christjani, Mark UNSPECIFIED UNSPECIFIED UNSPECIFIED Fink, Patrick UNSPECIFIED orcid.org/0000-0002-5927-8977 UNSPECIFIED von Elert, Eric UNSPECIFIED orcid.org/0000-0001-7758-716X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-273857
DOI:	10.1242/jeb.133504
Journal or Publication Title:	J. Exp. Biol.
Volume:	219
Number:	11
Page Range:	S. 1697 - 1705
Date:	2016
Publisher:	COMPANY BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	1477-9145
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language LIFE-HISTORY; JUVENILE-HORMONE; MORPHOLOGICAL DEFENSES; INDUCED POLYPHENISM; DEPTH SELECTION; CHAOBORUS; PULEX; BEHAVIOR; INDUCTION; DOPAMINE Multiple languages Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27385