Universität zu Köln

Schiavinato, Alvise, Keene, Douglas R., Wohl, Alexander P., Corallo, Diana ORCID: 0000-0001-7075-1414, Colombatti, Alfonso, Wagener, Raimund, Paulsson, Mats, Bonaldo, Paolo and Sengle, Gerhard (2016). Targeting of EMILIN-1 and EMILIN-2 to Fibrillin Microfibrils Facilitates their Incorporation into the Extracellular Matrix. J. Invest. Dermatol., 136 (6). S. 1150 - 1161. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1523-1747

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Abstract

Elastin microfibril interface-located proteins (EMILINs) 1 and 2 belong to a family of structurally related extracellular glycoproteins with unique functions in the extracellular space, such as modulation of pro-transforming growth factor-beta processing, activation of the extrinsic apoptotic pathway, and regulation of Hedgehog and Wnt ligand bioavailability. However, little is known about how EMILINs may exert their extracellular functions. We therefore investigated the spatiotemporal localization and deposition of EMILIN-1 and -2 within the extracellular space. By using immunoelectron and immunofluorescence microscopy together with biochemical extraction, we showed that EMILIN-1 and -2 are targeted to fibrillin microfibrils in the skin. In addition, during skin wound healing and in vitro matrix fiber assembly by primary dermal fibroblasts, EMILIN-1 and -2 are deposited on and coregulated with fibrillin. Analysis of wounds and mouse embryonic fibroblast cultures showed that EMILIN-1 and -2 network formation also requires the presence of fibronectin. Disruption of microfibrils in fibrillin-1edeficient mice leads to fragmentation of the EMILIN-1 and -2 networks, suggesting an involvement of EMILINs in fibrillin-related skin disorders. The addition of EMILINs to the ligand repertoire of fibrillin strengthens the concept of fibrillin microfibrils as extracellular scaffolds integrating cellular force transmission and growth factor bioactivity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schiavinato, Alvise UNSPECIFIED UNSPECIFIED UNSPECIFIED Keene, Douglas R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wohl, Alexander P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Corallo, Diana UNSPECIFIED orcid.org/0000-0001-7075-1414 UNSPECIFIED Colombatti, Alfonso UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagener, Raimund UNSPECIFIED UNSPECIFIED UNSPECIFIED Paulsson, Mats UNSPECIFIED UNSPECIFIED UNSPECIFIED Bonaldo, Paolo UNSPECIFIED UNSPECIFIED UNSPECIFIED Sengle, Gerhard UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-274077
DOI:	10.1016/j.jid.2016.02.021
Journal or Publication Title:	J. Invest. Dermatol.
Volume:	136
Number:	6
Page Range:	S. 1150 - 1161
Date:	2016
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1523-1747
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TGF-BETA; INTEGRIN INTERACTION; MARFAN-SYNDROME; EXPRESSION; GROWTH; MOUSE; GLYCOPROTEIN; PROTEIN; SKIN; INTERFACE Multiple languages Dermatology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27407