Universität zu Köln

Feike, Doreen, Seung, David ORCID: 0000-0003-3905-3647, Graf, Alexander ORCID: 0000-0002-6696-5206, Bischof, Sylvain, Ellick, Tamaryn, Coiro, Mario ORCID: 0000-0002-0113-0320, Soyk, Sebastian ORCID: 0000-0003-0325-4404, Eicke, Simona, Mettler-Altmann, Tabea, Lu, Kuan Jen, Trick, Martin, Zeeman, Samuel C. and Smith, Alison M. (2016). The Starch Granule-Associated Protein EARLY STARVATION1 Is Required for the Control of Starch Degradation in Arabidopsis thaliana Leaves. Plant Cell, 28 (6). S. 1472 - 1490. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1531-298X

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Abstract

To uncover components of the mechanism that adjusts the rate of leaf starch degradation to the length of the night, we devised a screen for mutant Arabidopsis thaliana plants in which starch reserves are prematurely exhausted. The mutation in one such mutant, named early starvation1 (esv1), eliminates a previously uncharacterized protein. Starch in mutant leaves is degraded rapidly and in a nonlinear fashion, so that reserves are exhausted 2 h prior to dawn. The ESV1 protein and a similar uncharacterized Arabidopsis protein (named Like ESV1 [LESV]) are located in the chloroplast stroma and are also bound into starch granules. The region of highest similarity between the two proteins contains a series of near-repeated motifs rich in tryptophan. Both proteins are conserved throughout starch-synthesizing organisms, from angiosperms and monocots to green algae. Analysis of transgenic plants lacking or overexpressing ESV1 or LESV, and of double mutants lacking ESV1 and another protein necessary for starch degradation, leads us to propose that these proteins function in the organization of the starch granule matrix. We argue that their misexpression affects starch degradation indirectly, by altering matrix organization and, thus, accessibility of starch polymers to starch-degrading enzymes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Feike, Doreen UNSPECIFIED UNSPECIFIED UNSPECIFIED Seung, David UNSPECIFIED orcid.org/0000-0003-3905-3647 UNSPECIFIED Graf, Alexander UNSPECIFIED orcid.org/0000-0002-6696-5206 UNSPECIFIED Bischof, Sylvain UNSPECIFIED UNSPECIFIED UNSPECIFIED Ellick, Tamaryn UNSPECIFIED UNSPECIFIED UNSPECIFIED Coiro, Mario UNSPECIFIED orcid.org/0000-0002-0113-0320 UNSPECIFIED Soyk, Sebastian UNSPECIFIED orcid.org/0000-0003-0325-4404 UNSPECIFIED Eicke, Simona UNSPECIFIED UNSPECIFIED UNSPECIFIED Mettler-Altmann, Tabea UNSPECIFIED UNSPECIFIED UNSPECIFIED Lu, Kuan Jen UNSPECIFIED UNSPECIFIED UNSPECIFIED Trick, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Zeeman, Samuel C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Smith, Alison M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-274828
DOI:	10.1105/tpc.16.00011
Journal or Publication Title:	Plant Cell
Volume:	28
Number:	6
Page Range:	S. 1472 - 1490
Date:	2016
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1531-298X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ADP-GLUCOSE PYROPHOSPHORYLASE; BRANCHING ENZYME; GENE-EXPRESSION; R1 PROTEIN; MUTANT; CHLOROPLAST; PHOSPHATASE; SYNTHASE; GLUCAN; TURNOVER Multiple languages Biochemistry & Molecular Biology; Plant Sciences; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27482