Universität zu Köln

Lane, Thomas S., Rempe, Caroline S., Davitt, Jack, Staton, Margaret E., Peng, Yanhui, Soltis, Douglas Edward, Melkonian, Michael, Deyholos, Michael, Leebens-Mack, James H., Chase, Mark, Rothfels, Carl J., Stevenson, Dennis, Graham, Sean W., Yu, Jun, Liu, Tao, Pires, J. Chris ORCID: 0000-0001-9682-2639, Edger, Patrick P., Zhang, Yong, Xie, Yinlong, Zhu, Ying, Carpenter, Eric, Wong, Gane Ka-Shu and Stewart, C. Neal, Jr. (2016). Diversity of ABC transporter genes across the plant kingdom and their potential utility in biotechnology. BMC Biotechnol., 16. LONDON: BMC. ISSN 1472-6750

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Abstract

Background: The ATP-binding cassette (ABC) transporter gene superfamily is ubiquitous among extant organisms and prominently represented in plants. ABC transporters act to transport compounds across cellular membranes and are involved in a diverse range of biological processes. Thus, the applicability to biotechnology is vast, including cancer resistance in humans, drug resistance among vertebrates, and herbicide and other xenobiotic resistance in plants. In addition, plants appear to harbor the highest diversity of ABC transporter genes compared with any other group of organisms. This study applied transcriptome analysis to survey the kingdom-wide ABC transporter diversity in plants and suggest biotechnology applications of this diversity. Results: We utilized sequence similarity-based informatics techniques to infer the identity of ABC transporter gene candidates from 1295 phylogenetically-diverse plant transcriptomes. A total of 97,149 putative (approximately 25 % were full-length) ABC transporter gene members were identified; each RNA-Seq library (plant sample) had 88 +/- 30 gene members. As expected, simpler organisms, such as algae, had fewer unique members than vascular land plants. Differences were also noted in the richness of certain ABC transporter subfamilies. Land plants had more unique ABCB, ABCC, and ABCG transporter gene members on average (p < 0.005), and green algae, red algae, and bryophytes had significantly more ABCF transporter gene members (p < 0.005). Ferns had significantly fewer ABCA transporter gene members than all other plant groups (p < 0.005). Conclusions: We present a transcriptomic overview of ABC transporter gene members across all major plant groups. An increase in the number of gene family members present in the ABCB, ABCC, and ABCD transporter subfamilies may indicate an expansion of the ABC transporter superfamily among green land plants, which include all crop species. The striking difference between the number of ABCA subfamily transporter gene members between ferns and other plant taxa is surprising and merits further investigation. Discussed is the potential exploitation of ABC transporters in plant biotechnology, with an emphasis on crops.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lane, Thomas S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Rempe, Caroline S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Davitt, Jack UNSPECIFIED UNSPECIFIED UNSPECIFIED Staton, Margaret E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Peng, Yanhui UNSPECIFIED UNSPECIFIED UNSPECIFIED Soltis, Douglas Edward UNSPECIFIED UNSPECIFIED UNSPECIFIED Melkonian, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Deyholos, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Leebens-Mack, James H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Chase, Mark UNSPECIFIED UNSPECIFIED UNSPECIFIED Rothfels, Carl J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Stevenson, Dennis UNSPECIFIED UNSPECIFIED UNSPECIFIED Graham, Sean W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Yu, Jun UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Tao UNSPECIFIED UNSPECIFIED UNSPECIFIED Pires, J. Chris UNSPECIFIED orcid.org/0000-0001-9682-2639 UNSPECIFIED Edger, Patrick P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhang, Yong UNSPECIFIED UNSPECIFIED UNSPECIFIED Xie, Yinlong UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhu, Ying UNSPECIFIED UNSPECIFIED UNSPECIFIED Carpenter, Eric UNSPECIFIED UNSPECIFIED UNSPECIFIED Wong, Gane Ka-Shu UNSPECIFIED UNSPECIFIED UNSPECIFIED Stewart, C. Neal, Jr. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-275119
DOI:	10.1186/s12896-016-0277-6
Journal or Publication Title:	BMC Biotechnol.
Volume:	16
Date:	2016
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	1472-6750
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BINDING CASSETTE TRANSPORTERS; HORSEWEED CONYZA-CANADENSIS; FUNCTIONAL CLASSIFICATION; EXPRESSION ANALYSIS; RESISTANCE; INITIATION; SUBFAMILY; SYSTEMS; EVOLUTION; PROTEINS Multiple languages Biotechnology & Applied Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27511