Universität zu Köln

Ludwig, Nicole, Leidinger, Petra, Becker, Kurt, Backes, Christina, Fehlmann, Tobias, Pallasch, Christian, Rheinheimer, Steffi, Meder, Benjamin, Staehler, Cord, Meese, Eckart and Keller, Andreas (2016). Distribution of miRNA expression across human tissues. Nucleic Acids Res., 44 (8). S. 3865 - 3878. OXFORD: OXFORD UNIV PRESS. ISSN 1362-4962

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Abstract

We present a human miRNA tissue atlas by determining the abundance of 1997 miRNAs in 61 tissue biopsies of different organs from two individuals collected post-mortem. One thousand three hundred sixty-four miRNAs were discovered in at least one tissue, 143 were present in each tissue. To define the distribution of miRNAs, we utilized a tissue specificity index (TSI). The majority of miRNAs (82.9%) fell in a middle TSI range i.e. were neither specific for single tissues (TSI > 0.85) nor housekeeping miRNAs (TSI < 0.5). Nonetheless, we observed many different miRNAs and miRNA families that were predominantly expressed in certain tissues. Clustering of miRNA abundances revealed that tissues like several areas of the brain clustered together. Considering -3p and -5p mature forms we observed miR-150 with different tissue specificity. Analysis of additional lung and prostate biopsies indicated that inter-organism variability was significantly lower than inter-organ variability. Tissue-specific differences between the miRNA patterns appeared not to be significantly altered by storage as shown for heart and lung tissue. MiRNAs TSI values of human tissues were significantly (P = 10(-8)) correlated with those of rats; miRNAs that were highly abundant in certain human tissues were likewise abundant in according rat tissues.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ludwig, Nicole UNSPECIFIED UNSPECIFIED UNSPECIFIED Leidinger, Petra UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Kurt UNSPECIFIED UNSPECIFIED UNSPECIFIED Backes, Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED Fehlmann, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Pallasch, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Rheinheimer, Steffi UNSPECIFIED UNSPECIFIED UNSPECIFIED Meder, Benjamin UNSPECIFIED UNSPECIFIED UNSPECIFIED Staehler, Cord UNSPECIFIED UNSPECIFIED UNSPECIFIED Meese, Eckart UNSPECIFIED UNSPECIFIED UNSPECIFIED Keller, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-275933
DOI:	10.1093/nar/gkw116
Journal or Publication Title:	Nucleic Acids Res.
Volume:	44
Number:	8
Page Range:	S. 3865 - 3878
Date:	2016
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1362-4962
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CIRCULATING MICRORNAS; RNA; BIOMARKERS; PROFILES; ATLAS; BLOOD; GENE; HEPATITIS; CARCINOMA; DISEASE Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27593