Universität zu Köln

Bradler, Cathleen, Warren, Ben ORCID: 0000-0002-7871-0091, Bardos, Viktor, Schleicher, Sabine, Klein, Andreas and Kloppenburg, Peter (2016). Properties and physiological function of Ca2+-dependent K+ currents in uniglomerular olfactory projection neurons. J. Neurophysiol., 115 (5). S. 2330 - 2341. BETHESDA: AMER PHYSIOLOGICAL SOC. ISSN 1522-1598

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Abstract

Ca2+-activated potassium currents [I-kappa(Ca)] are an important link between the intracellular signaling system and the membrane potential, which shapes intrinsic electro-physiological properties. To better understand the ionic mechanisms that mediate intrinsic firing properties of olfactory uniglomerular projection neurons (uPNs), we used whole cell patch-clamp recordings in an intact adult brain preparation of the male cockroach Periplaneta americana to analyze electrophysiological. In the insect brain, uPNs form the principal pathway from the antennal lobe to the protocerebrum, where centers for multimodal sensory processing and learning are located. In uPNs the activation of I-kappa(Ca) was clearly voltage and Ca2+ dependent. Thus under physiological conditions I-kappa(Ca) is strongly dependent on Ca2+ influx kinetics and on the membrane potential. The biophysical characterization suggests that I-kappa(Ca) is generated by big-conductance (BK) channels. A small-conductance (SK) channel-generated current could not be detected. I-kappa(Ca) was sensitive to charybdotoxin (CTX) and iberiotoxin (IbTX) but not to apamin. The functional role of I-kappa(Ca) was analyzed in occlusion experiments under current clamp, in which portions of I-kappa(Ca) were blocked by CTX or IbTX. Blockade of I-kappa(Ca) showed that I-kappa(Ca) contributes significantly to intrinsic electrophysiological properties such as the action potential waveform and membrane excitability.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Bradler, Cathleen UNSPECIFIED UNSPECIFIED UNSPECIFIED Warren, Ben UNSPECIFIED orcid.org/0000-0002-7871-0091 UNSPECIFIED Bardos, Viktor UNSPECIFIED UNSPECIFIED UNSPECIFIED Schleicher, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Klein, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Kloppenburg, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-276422
DOI:	10.1152/jn.00840.2015
Journal or Publication Title:	J. Neurophysiol.
Volume:	115
Number:	5
Page Range:	S. 2330 - 2341
Date:	2016
Publisher:	AMER PHYSIOLOGICAL SOC
Place of Publication:	BETHESDA
ISSN:	1522-1598
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ACTIVATED POTASSIUM CHANNELS; ANTENNAL LOBE NEURONS; SMALL-CONDUCTANCE; LOCAL INTERNEURONS; CA2+ CURRENTS; DIFFERENTIAL EXPRESSION; PERIPLANETA-AMERICANA; RECEPTOR NEURONS; MUSHROOM BODY; CALCIUM Multiple languages Neurosciences; Physiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27642