Universität zu Köln

Kvasnicka, Hans Michael ORCID: 0000-0002-3081-1395, Beham-Schmid, Christine, Bob, Roshanak, Dirnhofer, Stephan, Hussein, Kais, Kreipe, Hans, Kremer, Marcus, Schmitt-Graeff, Annette, Schwarz, Stephan, Thiele, Juergen, Werner, Martin and Stein, Harald (2016). Problems and pitfalls in grading of bone marrow fibrosis, collagen deposition and osteosclerosis - a consensus-based study. Histopathology, 68 (6). S. 905 - 916. HOBOKEN: WILEY. ISSN 1365-2559

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Abstract

Aims: In the era of potentially disease-modifying agents such as Janus kinase inhibitors, accurate grading and differentiation of bone marrow (BM) fibrosis has become more relevant to assess staging of disease and therapeutic effects. However, different fibrosis grading models have been used in the past without uniformity, including the proposal by the World Health Organization. Current scoring systems are based only on reticulin fibrosis. Therefore, additional assessment of collagen and the grade of osteosclerosis appear to be essential to discriminate all components of the complex BM fibrous matrix. Methods and results: We evaluated problems and pitfalls regarding staining techniques and the interpretation of reticulin fibrosis on a total of 352 samples. Furthermore, we propose a minor modification of the current grading and separate scoring for collagen deposition and osteosclerosis. Reproducibility of gradings was tested among 11 haematopathologists in a blinded assessment. Overall, the inter-rater reliability of all three grading systems ranged between 0.898 and 0.926. Conclusions: A standardized assessment of BM fibrosis with differentiation between reticulin, collagen and osteosclerosis is recommended to evaluate the various components of the fibrous matrix which may be delinked after therapy. In this regard, quality of staining and application of laboratory standards enable a highly reproducible scoring.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kvasnicka, Hans Michael UNSPECIFIED orcid.org/0000-0002-3081-1395 UNSPECIFIED Beham-Schmid, Christine UNSPECIFIED UNSPECIFIED UNSPECIFIED Bob, Roshanak UNSPECIFIED UNSPECIFIED UNSPECIFIED Dirnhofer, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Hussein, Kais UNSPECIFIED UNSPECIFIED UNSPECIFIED Kreipe, Hans UNSPECIFIED UNSPECIFIED UNSPECIFIED Kremer, Marcus UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmitt-Graeff, Annette UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwarz, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Thiele, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Werner, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Stein, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-277017
DOI:	10.1111/his.12871
Journal or Publication Title:	Histopathology
Volume:	68
Number:	6
Page Range:	S. 905 - 916
Date:	2016
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-2559
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CHRONIC IDIOPATHIC MYELOFIBROSIS; CHRONIC MYELOPROLIFERATIVE DISORDERS; SILVER IMPREGNATION; RETICULIN FIBROSIS; ESSENTIAL THROMBOCYTHEMIA; RISK-STRATIFICATION; MYELOID METAPLASIA; EUROPEAN CONSENSUS; DIAGNOSIS; CLASSIFICATION Multiple languages Cell Biology; Pathology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27701