Universität zu Köln

Kalimuthu, Palraj, Belaidi, Abdel A., Schwarz, Guenter ORCID: 0000-0002-2118-9338 and Bernhardt, Paul V. (2016). Low Potential Catalytic Voltammetry of Human Sulfite Oxidase. Electrochim. Acta, 199. S. 280 - 290. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 1873-3859

Full text not available from this repository.

Abstract

Mediated electrocatalytic voltammetry of human sulfite oxidase (HSO) is demonstrated with synthetic one electron transfer iron complexes bis(1,4,7-triazacyclononane)iron(III) ([Fe(tacn)(2)](3+)) and 1,2-bis (1,4,7-triaza-1-cyclononyl)ethane iron(III) ([Fe(dtne)](3+)) at a glassy carbon working electrode. The two synthetic electron acceptors for HSO, differing in redox potential by 270 mV, deliver different driving forces for electrocatalysis. Digital simulation of the catalytic voltammetry was achieved with single set of enzyme-dependent kinetic parameters that reproduced the experimental data across a range of sweep rates, and sulfite and mediator concentrations. Amperometry carried out in a stirred solution with the lower potential mediator [Fe(tacn)(2)](3+) was optimised and exhibited a linear increase in steady state current in the sulfite concentration range 5.0 x 10(-6) to 8.0 x 10(-4) M with a detection limit of 0.2 pM (S/N = 3). The HSO coupled electrode was successfully used for the determination of sulfite concentration in white wine and beer samples and the results validated with a standard spectrophotometric method. (C) 2016 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kalimuthu, Palraj UNSPECIFIED UNSPECIFIED UNSPECIFIED Belaidi, Abdel A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwarz, Guenter UNSPECIFIED orcid.org/0000-0002-2118-9338 UNSPECIFIED Bernhardt, Paul V. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-277190
DOI:	10.1016/j.electacta.2016.01.181
Journal or Publication Title:	Electrochim. Acta
Volume:	199
Page Range:	S. 280 - 290
Date:	2016
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	1873-3859
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INTRAMOLECULAR ELECTRON-TRANSFER; MODIFIED GOLD ELECTRODES; DIRECT ELECTROCHEMISTRY; MOLYBDENUM ENZYMES; OXIDIZING ENZYMES; CYTOCHROME-C; DEHYDROGENASE; DEFICIENCY; COMPLEXES; PROTEIN Multiple languages Electrochemistry Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27719