Universität zu Köln

Westermayer, Sonja A., Fritz, Georg ORCID: 0000-0002-1503-9165, Gutierrez, Joaquin, Megerle, Judith A., Weissl, Mira P. S., Schnetz, Karin ORCID: 0000-0003-4693-6202, Gerland, Ulrich ORCID: 0000-0002-0859-6422 and Raedler, Joachim O. (2016). Single-cell characterization of metabolic switching in the sugar phosphotransferase system of Escherichia coli. Mol. Microbiol., 100 (3). S. 472 - 486. HOBOKEN: WILEY. ISSN 1365-2958

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Abstract

The utilization of several sugars in Escherichia coli is regulated by the Phosphotransferase System (PTS), in which diverse sugar utilization modules compete for phosphoryl flux from the general PTS proteins. Existing theoretical work predicts a winner-take-all outcome when this flux limits carbon uptake. To date, no experimental work has interrogated competing PTS uptake modules with single-cell resolution. Using time-lapse microscopy in perfused microchannels, we analyzed the competition between N-acetyl-glucosamine and sorbitol, as representative PTS sugars, by measuring both the expression of their utilization systems and the concomitant impact of sugar utilization on growth rates. We find two distinct regimes: hierarchical usage of the carbohydrates, and co-expression of the genes for both systems. Simulations of a mathematical model incorporating asymmetric sugar quality reproduce our metabolic phase diagram, indicating that under conditions of nonlimiting phosphate flux, co-expression is due to uncoupling of both sugar utilization systems. Our model reproduces hierarchical winner-take-all behaviour and stochastic co-expression, and predicts the switching between both strategies as a function of available phosphate flux. Hence, experiments and theory both suggest that PTS sugar utilization involves not only switching between the sugars utilized but also switching of utilization strategies to accommodate prevailing environmental conditions.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Westermayer, Sonja A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Fritz, Georg UNSPECIFIED orcid.org/0000-0002-1503-9165 UNSPECIFIED Gutierrez, Joaquin UNSPECIFIED UNSPECIFIED UNSPECIFIED Megerle, Judith A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Weissl, Mira P. S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schnetz, Karin UNSPECIFIED orcid.org/0000-0003-4693-6202 UNSPECIFIED Gerland, Ulrich UNSPECIFIED orcid.org/0000-0002-0859-6422 UNSPECIFIED Raedler, Joachim O. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-277270
DOI:	10.1111/mmi.13329
Journal or Publication Title:	Mol. Microbiol.
Volume:	100
Number:	3
Page Range:	S. 472 - 486
Date:	2016
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-2958
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CARBON CATABOLITE REPRESSION; NAG REGULON; BACTERIAL PHOSPHOENOLPYRUVATE; PROTEIN-PHOSPHORYLATION; N-ACETYLGLUCOSAMINE; INDUCER EXCLUSION; ARABINOSE; EXPRESSION; GROWTH; INDUCTION Multiple languages Biochemistry & Molecular Biology; Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27727