Universität zu Köln

Zhou, Zhigang, Pausch, Friederike, Schloetzer-Schrehardt, Ursula, Brachvogel, Bent and Poeschl, Ernst (2016). Induction of initial steps of angiogenic differentiation and maturation of endothelial cells by pericytes in vitro and the role of collagen IV. Histochem. Cell Biol., 145 (5). S. 511 - 526. NEW YORK: SPRINGER. ISSN 1432-119X

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Abstract

Activation of endothelial cells and recruitment of mural cells define critical steps during the formation of stable vascular elements. Both events are reflected by cocultures of endothelial cells and isolated murine pericyte-like cells and define a versatile platform for the analysis of distinct steps during the angiogenic process in vitro. Isolated pericyte-like cells promote the survival of endothelial cells, induce the assembly of endothelial cells as well as establish direct contacts with forming endothelial alignments. More importantly, they also induce characteristic steps of maturation including the assembly of stable cell-cell junctions, deposition of basement membrane-like matrices and local formation of a central lumen. The presence of pericyte-like cells induces the secretion of extracellular matrices enriched in collagen IV by endothelial cells, which improves endothelial tube formation and provides the adhesive substrate for mural cell recruitment. Collagen-binding integrins contribute differentially to the process, with alpha 1 beta 1 involved in the adhesion of pericyte-like cells to collagen IV and alpha 2 beta 1 mainly involved in endothelial cord formation. These data indicate that pericyte-like cells are essential for the survival of endothelial cells, the efficient formation of endothelial alignments as well as initial steps of maturation of capillary-like structures.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zhou, Zhigang UNSPECIFIED UNSPECIFIED UNSPECIFIED Pausch, Friederike UNSPECIFIED UNSPECIFIED UNSPECIFIED Schloetzer-Schrehardt, Ursula UNSPECIFIED UNSPECIFIED UNSPECIFIED Brachvogel, Bent UNSPECIFIED UNSPECIFIED UNSPECIFIED Poeschl, Ernst UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-277710
DOI:	10.1007/s00418-015-1398-z
Journal or Publication Title:	Histochem. Cell Biol.
Volume:	145
Number:	5
Page Range:	S. 511 - 526
Date:	2016
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-119X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HEPARAN-SULFATE; ALPHA(2)BETA(1) INTEGRINS; PERIVASCULAR CELLS; DEFICIENT MOUSE; ANNEXIN A5; PDGF-B; MATRIX; GROWTH; MORPHOGENESIS; EXPRESSION Multiple languages Cell Biology; Microscopy Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27771