Universität zu Köln

Liu, Yansheng ORCID: 0000-0002-2626-3912, Beyer, Andreas ORCID: 0000-0002-3891-2123 and Aebersold, Ruedi (2016). On the Dependency of Cellular Protein Levels on mRNA Abundance. Cell, 165 (3). S. 535 - 551. CAMBRIDGE: CELL PRESS. ISSN 1097-4172

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Abstract

The question of how genomic information is expressed to determine phenotypes is of central importance for basic and translational life science research and has been studied by transcriptomic and proteomic profiling. Here, we review the relationship between protein and mRNA levels under various scenarios, such as steady state, long-term state changes, and short-term adaptation, demonstrating the complexity of gene expression regulation, especially during dynamic transitions. The spatial and temporal variations of mRNAs, as well as the local availability of resources for protein biosynthesis, strongly influence the relationship between protein levels and their coding transcripts. We further discuss the buffering of mRNA fluctuations at the level of protein concentrations. We conclude that transcript levels by themselves are not sufficient to predict protein levels in many scenarios and to thus explain genotype-phenotype relationships and that high-quality data quantifying different levels of gene expression are indispensable for the complete understanding of biological processes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Liu, Yansheng UNSPECIFIED orcid.org/0000-0002-2626-3912 UNSPECIFIED Beyer, Andreas UNSPECIFIED orcid.org/0000-0002-3891-2123 UNSPECIFIED Aebersold, Ruedi UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-278384
DOI:	10.1016/j.cell.2016.03.014
Journal or Publication Title:	Cell
Volume:	165
Number:	3
Page Range:	S. 535 - 551
Date:	2016
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1097-4172
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language QUANTITATIVE PROTEOMIC ANALYSIS; GENOME-WIDE ANALYSIS; GENE-EXPRESSION; SACCHAROMYCES-CEREVISIAE; ABSOLUTE PROTEIN; MASS-SPECTROMETRY; GLOBAL ANALYSIS; COPY-NUMBER; IN-VIVO; REGULATORY VARIATION Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27838