Universität zu Köln

Hampel, Martin, Jakobi, Mareike, Schmitz, Lara, Meyer, Ute, Finkernagel, Florian, Doehlemann, Gunther ORCID: 0000-0002-7353-8456 and Heimel, Kai ORCID: 0000-0002-1396-8622 (2016). Unfolded Protein Response (UPR) Regulator Cib1 Controls Expression of Genes Encoding Secreted Virulence Factors in Ustilago maydis. PLoS One, 11 (4). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

The unfolded protein response (UPR), a conserved eukaryotic signaling pathway to ensure protein homeostasis in the endoplasmic reticulum (ER), coordinates biotrophic development in the corn smut fungus Ustilago maydis. Exact timing of UPR activation is required for virulence and presumably connected to the elevated expression of secreted effector proteins during infection of the host plant Zea mays. In the baker's yeast Saccharomyces cerevisiae, expression of UPR target genes is induced upon binding of the central regulator Hac1 to unfolded protein response elements (UPREs) in their promoters. While a role of the UPR in effector secretion has been described previously, we investigated a potential UPR-dependent regulation of genes encoding secreted effector proteins. In silico prediction of UPREs in promoter regions identified the previously characterized effector genes pit2 and tin1-1, as bona fide UPR target genes. Furthermore, direct binding of the Hac1-homolog Cib1 to the UPRE containing promoter fragments of both genes was confirmed by quantitative chromatin immunoprecipitation (qChIP) analysis. Targeted deletion of the UPRE abolished Cib1-dependent expression of pit2 and significantly affected virulence. Furthermore, ER stress strongly increased Pit2 expression and secretion. This study expands the role of the UPR as a signal hub in fungal virulence and illustrates, how biotrophic fungi can coordinate cellular physiology, development and regulation of secreted virulence factors.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hampel, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Jakobi, Mareike UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmitz, Lara UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyer, Ute UNSPECIFIED UNSPECIFIED UNSPECIFIED Finkernagel, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Doehlemann, Gunther UNSPECIFIED orcid.org/0000-0002-7353-8456 UNSPECIFIED Heimel, Kai UNSPECIFIED orcid.org/0000-0002-1396-8622 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-278488
DOI:	10.1371/journal.pone.0153861
Journal or Publication Title:	PLoS One
Volume:	11
Number:	4
Date:	2016
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PATHOGENIC DEVELOPMENT; TRANSCRIPTION FACTOR; ENDOPLASMIC-RETICULUM; REPLACEMENT MUTANTS; FUNGAL EFFECTOR; MESSENGER-RNA; CLP1 PROTEIN; ER-STRESS; IRE1; GENOME Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27848