Universität zu Köln

Shahbazi, Ebrahim, Moradi, Sharif ORCID: 0000-0002-2935-983X, Nemati, Shiva, Satarian, Leila ORCID: 0000-0002-5068-5031, Basiri, Mohsen ORCID: 0000-0003-2512-7641, Gourabi, Hamid, Mehrjardi, Narges Zare, Guenther, Patrick, Lampert, Angelika ORCID: 0000-0001-6319-6272, Haendler, Kristian, Hatay, Firuze Fulya, Schmidt, Diana, Molcanyi, Marek, Hescheler, Juergen, Schultze, Joachim L., Saric, Tomo ORCID: 0000-0001-8344-1095 and Baharvand, Hossein (2016). Conversion of Human Fibroblasts to Stably Self-Renewing Neural Stem Cells with a Single Zinc-Finger Transcription Factor. Stem Cell Rep., 6 (4). S. 539 - 552. CAMBRIDGE: CELL PRESS. ISSN 2213-6711

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Abstract

Direct conversion of somatic cells into neural stem cells (NSCs) by defined factors holds great promise for mechanistic studies, drug screening, and potential cell therapies for different neurodegenerative diseases. Here, we report that a single zinc-finger transcription factor, Zfp521, is sufficient for direct conversion of human fibroblasts into long-term self-renewable and multipotent NSCs. In vitro, Zfp521-induced NSCs maintained their characteristics in the absence of exogenous factor expression and exhibited morphological, molecular, developmental, and functional properties that were similar to control NSCs. In addition, the single-seeded induced NSCs were able to form NSC colonies with efficiency comparable with control NSCs and expressed NSC markers. The converted cells were capable of surviving, migrating, and attaining neural phenotypes after transplantation into neonatal mouse and adult rat brains, without forming tumors. Moreover, the Zfp521-induced NSCs predominantly expressed rostral genes. Our results suggest a facilitated approach for establishing human NSCs through Zfp521-driven conversion of fibroblasts.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Shahbazi, Ebrahim UNSPECIFIED UNSPECIFIED UNSPECIFIED Moradi, Sharif UNSPECIFIED orcid.org/0000-0002-2935-983X UNSPECIFIED Nemati, Shiva UNSPECIFIED UNSPECIFIED UNSPECIFIED Satarian, Leila UNSPECIFIED orcid.org/0000-0002-5068-5031 UNSPECIFIED Basiri, Mohsen UNSPECIFIED orcid.org/0000-0003-2512-7641 UNSPECIFIED Gourabi, Hamid UNSPECIFIED UNSPECIFIED UNSPECIFIED Mehrjardi, Narges Zare UNSPECIFIED UNSPECIFIED UNSPECIFIED Guenther, Patrick UNSPECIFIED UNSPECIFIED UNSPECIFIED Lampert, Angelika UNSPECIFIED orcid.org/0000-0001-6319-6272 UNSPECIFIED Haendler, Kristian UNSPECIFIED UNSPECIFIED UNSPECIFIED Hatay, Firuze Fulya UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Diana UNSPECIFIED UNSPECIFIED UNSPECIFIED Molcanyi, Marek UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Schultze, Joachim L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Saric, Tomo UNSPECIFIED orcid.org/0000-0001-8344-1095 UNSPECIFIED Baharvand, Hossein UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-278708
DOI:	10.1016/j.stemcr.2016.02.013
Journal or Publication Title:	Stem Cell Rep.
Volume:	6
Number:	4
Page Range:	S. 539 - 552
Date:	2016
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2213-6711
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DIRECT LINEAGE CONVERSION; FUNCTIONAL-NEURONS; DEFINED FACTORS; MOUSE; DIFFERENTIATION; INTEGRATION; PROGENITORS; GENERATION; FATE Multiple languages Cell & Tissue Engineering; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27870