Universität zu Köln

Braun, Fabian ORCID: 0000-0002-4742-050X, Rinschen, Markus M., Bartels, Valerie, Frommolt, Peter ORCID: 0000-0002-1966-8014, Habermann, Bianca ORCID: 0000-0002-2457-7504, Hoeijmakers, Jan H. J., Schumacher, Bojrn, Dolle, Martijn E. T., Mueller, Roman-Ulrich, Benzing, Thomas, Schermer, Bernhard ORCID: 0000-0002-5194-9000 and Kurschat, Christine E. (2016). Altered lipid metabolism in the aging kidney identified by three layered omic analysis. Aging-US, 8 (3). S. 441 - 458. ORCHARD PARK: IMPACT JOURNALS LLC. ISSN 1945-4589

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Abstract

Aging-associated diseases and their comorbidities affect the life of a constantly growing proportion of the population in developed countries. At the center of these comorbidities are changes of kidney structure and function as age-related chronic kidney disease predisposes to the development of cardiovascular diseases such as stroke, myocardial infarction or heart failure. To detect molecular mechanisms involved in kidney aging, we analyzed gene expression profiles of kidneys from adult and aged wild-type mice by transcriptomic, proteomic and targeted lipidomic methodologies. Interestingly, transcriptome and proteome analyses revealed differential expression of genes primarily involved in lipid metabolism and immune response. Additional lipidomic analyses uncovered significant age-related differences in the total amount of phosphatidylethanolamines, phosphatidylcholines and sphingomyelins as well as in subspecies of phosphatidylserines and ceramides with age. By integration of these datasets we identified Aldh1a1, a key enzyme in vitamin A metabolism specifically expressed in the medullary ascending limb, as one of the most prominent upregulated proteins in old kidneys. Moreover, ceramidase Asah1 was highly expressed in aged kidneys, consistent with a decrease in ceramide C16. In summary, our data suggest that changes in lipid metabolism are involved in the process of kidney aging and in the development of chronic kidney disease.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Braun, Fabian UNSPECIFIED orcid.org/0000-0002-4742-050X UNSPECIFIED Rinschen, Markus M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bartels, Valerie UNSPECIFIED UNSPECIFIED UNSPECIFIED Frommolt, Peter UNSPECIFIED orcid.org/0000-0002-1966-8014 UNSPECIFIED Habermann, Bianca UNSPECIFIED orcid.org/0000-0002-2457-7504 UNSPECIFIED Hoeijmakers, Jan H. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schumacher, Bojrn UNSPECIFIED UNSPECIFIED UNSPECIFIED Dolle, Martijn E. T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Roman-Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Benzing, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Schermer, Bernhard UNSPECIFIED orcid.org/0000-0002-5194-9000 UNSPECIFIED Kurschat, Christine E. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-283532
DOI:	10.18632/aging.100900
Journal or Publication Title:	Aging-US
Volume:	8
Number:	3
Page Range:	S. 441 - 458
Date:	2016
Publisher:	IMPACT JOURNALS LLC
Place of Publication:	ORCHARD PARK
ISSN:	1945-4589
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXPRESSION; LONGEVITY; CERAMIDE; INJURY; AGE; TRANSCRIPTOME; METABOLOMICS; REGULATOR; SOFTWARE; TISSUE Multiple languages Cell Biology; Geriatrics & Gerontology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28353