Universität zu Köln

Schoenle, Alexandra, Jeuck, Alexandra, Nitsche, Frank, Venter, Paul, Prausse, Dennis and Arndt, Hartmut ORCID: 0000-0003-2811-3595 (2016). Methodological Studies on Estimates of Abundance and Diversity of Heterotrophic Flagellates from the Deep-Sea Floor. J. Mar. Sci. Eng., 4 (1). BASEL: MDPI. ISSN 2077-1312

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Abstract

Extreme environmental conditions in the deep sea hamper access to protist communities. In combination with the potentially highly diverse species composition, it demands a wide range of methods to be applied at the same time to guarantee a high resolution of quantitative and qualitative studies of deep-sea heterotrophic flagellates (HF). Within this study, we present a possible combination of several culture-independent and culture-dependent methods available for investigating benthic deep-sea HF communities. Besides live-counting and fixation of HF, we refer to cultivation methods and molecular surveys using next generation sequencing. Laboratory ecological experiments under deep-sea conditions (high pressure, low temperature) could allow the approval of the potential deep-sea origin of sampled HF. The combination of different methods offers a unique possibility to receive detailed information on nanofaunal life in the deep sea. Specific fixation techniques to preserve samples directly at the sampling depth must be applied in further studies to reflect the real biodiversity of the largest habitat on earth.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schoenle, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Jeuck, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Nitsche, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Venter, Paul UNSPECIFIED UNSPECIFIED UNSPECIFIED Prausse, Dennis UNSPECIFIED UNSPECIFIED UNSPECIFIED Arndt, Hartmut UNSPECIFIED orcid.org/0000-0003-2811-3595 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-283831
DOI:	10.3390/jmse4010022
Journal or Publication Title:	J. Mar. Sci. Eng.
Volume:	4
Number:	1
Date:	2016
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2077-1312
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IN-SITU HYBRIDIZATION; QUANTITATIVE ASSESSMENT; EUKARYOTIC DIVERSITY; MICROBIAL EUKARYOTES; MEDITERRANEAN SEA; SURFACE WATERS; SEDIMENTS; PATTERNS; IDENTIFICATION; NANOPLANKTON Multiple languages Oceanography Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28383