Universität zu Köln

Lu, Yameng, Wohlrab, Sylke ORCID: 0000-0003-3190-0880, Groth, Marco ORCID: 0000-0002-9199-8990, Gloeckner, Gernot, Guillou, Laure ORCID: 0000-0003-1032-7958 and John, Uwe (2016). Transcriptomic profiling of Alexandrium fundyense during physical interaction with or exposure to chemical signals from the parasite Amoebophrya. Mol. Ecol., 25 (6). S. 1294 - 1308. HOBOKEN: WILEY. ISSN 1365-294X

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Abstract

Toxic microalgae have their own pathogens, and understanding the way in which these microalgae respond to antagonistic attacks may provide information about their capacity to persist during harmful algal bloom events. Here, we compared the effects of the physical presence of the parasite Amoebophrya sp. and exposure to waterborne cues from cultures infected with this parasite, on gene expression by the toxic dinoflagellates, Alexandrium fundyense. Compared with control samples, a total of 14882 Alexandrium genes were differentially expressed over the whole-parasite infection cycle at three different time points (0, 6 and 96h). RNA sequencing analyses indicated that exposure to the parasite and parasitic waterborne cues produced significant changes in the expression levels of Alexandrium genes associated with specific metabolic pathways. The observed upregulation of genes associated with glycolysis, the tricarboxylic acid cycle, fatty acid -oxidation, oxidative phosphorylation and photosynthesis suggests that parasite infection increases the energy demand of the host. The observed upregulation of genes correlated with signal transduction indicates that Alexandrium could be sensitized by parasite attacks. This response might prime the defence of the host, as indicated by the increased expression of several genes associated with defence and stress. Our findings provide a molecular overview of the response of a dinoflagellate to parasite infection.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lu, Yameng UNSPECIFIED UNSPECIFIED UNSPECIFIED Wohlrab, Sylke UNSPECIFIED orcid.org/0000-0003-3190-0880 UNSPECIFIED Groth, Marco UNSPECIFIED orcid.org/0000-0002-9199-8990 UNSPECIFIED Gloeckner, Gernot UNSPECIFIED UNSPECIFIED UNSPECIFIED Guillou, Laure UNSPECIFIED orcid.org/0000-0003-1032-7958 UNSPECIFIED John, Uwe UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-283934
DOI:	10.1111/mec.13566
Journal or Publication Title:	Mol. Ecol.
Volume:	25
Number:	6
Page Range:	S. 1294 - 1308
Date:	2016
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-294X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DINOFLAGELLATE ALEXANDRIUM; OXIDATIVE STRESS; CHESAPEAKE BAY; MARINE; HOST; TAMARENSE; EXPRESSION; INFECTION; STRAINS; DEFENSE Multiple languages Biochemistry & Molecular Biology; Ecology; Evolutionary Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28393