Universität zu Köln

Gersing, Alexandra S., Faymonville, Andrea M., Schwaiger, Benedikt J. ORCID: 0000-0003-1803-1510, Leypoldt, Frank, Siemonsen, Susanne, Zimmer, Claus, Magnus, Tim and Fiehler, Jens (2016). Quantitative T2*mapping reveals early temporo-spatial dynamics in an ischemic stroke model. J. Neurosci. Methods, 259. S. 83 - 90. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1872-678X

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Abstract

Background: Oxyhemoglobin-sensitive sequences, namely T2*, can indirectly depict changes in oxygen extraction. Purpose of this study was to investigate the dynamics of T2* changes in ischemic tissue. New method: We investigated earliest temporo-spatial dynamics within ischemic tissue, measured with quantitative T2* imaging in the histologically defined infarct core and surrounding surviving tissue. Middle cerebral artery occlusion (MCAO) was induced by a filament model in mice. Serial multiple gradient-echo T2* sequences and diffusion-weighted images were acquired for 60 min after MCAO and repeated for 60 min after recanalization. T2* maps were co-registered with histology and T2* changes were compared to the contralateral hemisphere. Results: Within the histologically defined infarct core, relative T2* values decreased significantly by -10.8 +/- 2.8% (P = 0.003) compared to the contralateral hemisphere within 3.5 +/- 0.7 min after MCAO. Relative T2* values in volume exceeding the histologically determined infarct core were significantly less decreased (-6.7 +/- 2.1%; P = 0.02) and increased after recanalization (+3.9 +/- 1.9%; P = 0.045). Volume with T2* decrease showed continuous growth over 60 min after MCAO (P = 0.002) and decreased during 60 min after recanalization (P = 0.026), showing most significant correlations between infarct core volume and T2* abnormality volume (r = 0.66; P = 0.037) of the last image acquired after recanalization. Comparison with existing method(s): To our best knowledge, this is the first application of non-invasive quantitative T2* measurements to assess changes in levels of deoxyhemoglobin as an indirect biomarker for metabolic impairment in ischemic tissue. Conclusions: Quantitative T2* imaging might be a feasible tool to indicate change of oxygenation in acute stroke imaging, without administration of contrast agent. (C) 2015 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gersing, Alexandra S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Faymonville, Andrea M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwaiger, Benedikt J. UNSPECIFIED orcid.org/0000-0003-1803-1510 UNSPECIFIED Leypoldt, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Siemonsen, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Zimmer, Claus UNSPECIFIED UNSPECIFIED UNSPECIFIED Magnus, Tim UNSPECIFIED UNSPECIFIED UNSPECIFIED Fiehler, Jens UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-286493
DOI:	10.1016/j.jneumeth.2015.11.018
Journal or Publication Title:	J. Neurosci. Methods
Volume:	259
Page Range:	S. 83 - 90
Date:	2016
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	1872-678X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CEREBRAL-ARTERY OCCLUSION; IMAGING RELAXATION-TIME; BLOOD OXYGENATION; GRADIENT-ECHO; HIGH-FIELD; RAT-BRAIN; MRI; PERFUSION; DIFFUSION; PENUMBRA Multiple languages Biochemical Research Methods; Neurosciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28649