Universität zu Köln

Krupina, Ksenia, Kleiss, Charlotte, Metzger, Thibaud, Fournane, Sadek, Schmucker, Stephane, Hofmann, Kay ORCID: 0000-0002-2289-9083, Fischer, Benoit, Paul, Nicodeme, Porter, Iain Malcolm, Raffelsberger, Wolfgang, Poch, Olivier, Swedlow, Jason Reese, Brino, Laurent and Sumara, Izabela ORCID: 0000-0002-5968-3358 (2016). Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules. Dev. Cell, 36 (1). S. 63 - 79. CAMBRIDGE: CELL PRESS. ISSN 1878-1551

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Abstract

Mitosis ensures equal segregation of the genome and is controlled by a variety of ubiquitylation signals on substrate proteins. However, it remains unexplored how the versatile ubiquitin code is read out during mitotic progression. Here, we identify the ubiquitin receptor protein UBASH3B as an important regulator of mitosis. UBASH3B interacts with ubiquitylated Aurora B, one of the main kinases regulating chromosome segregation, and controls its subcellular localization but not protein levels. UBASH3B is a limiting factor in this pathway and is sufficient to localize Aurora B to microtubules prior to anaphase. Importantly, targeting Aurora B to microtubules by UBASH3B is necessary for the timing and fidelity of chromosome segregation in human cells. Our findings uncover an important mechanism defining how ubiquitin attachment to a substrate protein is decoded during mitosis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Krupina, Ksenia UNSPECIFIED UNSPECIFIED UNSPECIFIED Kleiss, Charlotte UNSPECIFIED UNSPECIFIED UNSPECIFIED Metzger, Thibaud UNSPECIFIED UNSPECIFIED UNSPECIFIED Fournane, Sadek UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmucker, Stephane UNSPECIFIED UNSPECIFIED UNSPECIFIED Hofmann, Kay UNSPECIFIED orcid.org/0000-0002-2289-9083 UNSPECIFIED Fischer, Benoit UNSPECIFIED UNSPECIFIED UNSPECIFIED Paul, Nicodeme UNSPECIFIED UNSPECIFIED UNSPECIFIED Porter, Iain Malcolm UNSPECIFIED UNSPECIFIED UNSPECIFIED Raffelsberger, Wolfgang UNSPECIFIED UNSPECIFIED UNSPECIFIED Poch, Olivier UNSPECIFIED UNSPECIFIED UNSPECIFIED Swedlow, Jason Reese UNSPECIFIED UNSPECIFIED UNSPECIFIED Brino, Laurent UNSPECIFIED UNSPECIFIED UNSPECIFIED Sumara, Izabela UNSPECIFIED orcid.org/0000-0002-5968-3358 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-287379
DOI:	10.1016/j.devcel.2015.12.017
Journal or Publication Title:	Dev. Cell
Volume:	36
Number:	1
Page Range:	S. 63 - 79
Date:	2016
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1878-1551
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CHROMOSOMAL PASSENGER COMPLEX; SPINDLE-ASSEMBLY CHECKPOINT; HUMAN-CELLS; BINDING PROTEINS; TYROSINE KINASES; MITOSIS; ACTIVATION; CYTOKINESIS; RELOCATION; REQUIRES Multiple languages Cell Biology; Developmental Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28737