Universität zu Köln

Peters, Ulrike, Solominidou, Eleni, Korkmaz, Yueksel, Ruettermann, Stefan, Klocke, Astrid, Flemmig, Thomas Frank and Beikler, Thomas (2016). Regulator of Calcineurin 1 in Periodontal Disease. Mediat. Inflamm., 2016. LONDON: HINDAWI LTD. ISSN 1466-1861

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Abstract

Nuclear factor of activated T-cells (NFAT) and NF-kB pathway associated processes are involved in the pathogenesis of various inflammatory disorders, for example, periodontal disease. The activation of these pathways is controlled by the regulator of calcineurin 1 (RCAN1). The aim of this study was to elucidate the role of RCAN1 in periodontal disease. Healthy and inflamed periodontal tissues were analyzed by immunohistochemistry and immunofluorescence using specific rabbit polyclonal anti-RCAN1 antibodies. For expression analysis human umbilical vein endothelial cells (HUVEC) were used. HUVEC were incubated for 2h with Vascular Endothelial Growth Factor (VEGF) or with wild type and laboratory strains of Porphyromonas gingivalis (P. gingivalis). Expression analysis of rcan1 and cox2 was done by real time PCR using specific primers for rcan1.4 and cox2. The expression of rcan1 was found to be significantly suppressed in endothelial cells of chronically inflamed periodontal tissues compared to healthy controls. Rcan1 and cox2 were significantly induced by VEGF and wild type and laboratory P. gingivalis strains. Interestingly, the magnitude of the rcan1 and cox2 induction was strain dependent. The results of this study indicate that RCAN1 is suppressed in endothelial cells of chronically inflamed periodontal tissues. During an acute infection, however, rcan1 seems to be upregulated in endothelial cells, indicating a modulating role in immune homeostasis of periodontal tissues.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Peters, Ulrike UNSPECIFIED UNSPECIFIED UNSPECIFIED Solominidou, Eleni UNSPECIFIED UNSPECIFIED UNSPECIFIED Korkmaz, Yueksel UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruettermann, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Klocke, Astrid UNSPECIFIED UNSPECIFIED UNSPECIFIED Flemmig, Thomas Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Beikler, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-289686
DOI:	10.1155/2016/5475821
Journal or Publication Title:	Mediat. Inflamm.
Volume:	2016
Date:	2016
Publisher:	HINDAWI LTD
Place of Publication:	LONDON
ISSN:	1466-1861
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ACTIVATED T-CELLS; DOWN-SYNDROME; GENE-EXPRESSION; RCAN1; DSCR1; INFLAMMATION; INHIBITOR; CALCIUM; FAMILY; PHOSPHORYLATION Multiple languages Cell Biology; Immunology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28968