Gassa, Asmae, Kalkavan, Halime ORCID: 0000-0002-0090-4080, Jian, Fu, Duhan, Vikas ORCID: 0000-0002-6337-8225, Khairnar, Vishal ORCID: 0000-0002-3584-7530, Shaabani, Namir, Honke, Nadine, Carpinteiro, Alexander, Botezatu, Lacrarnioara, Crivello, Pietro, Dolff, Sebastian ORCID: 0000-0001-5549-1706, Ferencik, Stanislav, Haussinger, Dieter, Khandanpour, Cyrus ORCID: 0000-0003-4655-6269, Fleischhauer, Katharina, Witzke, Oliver, Wahlers, Thorsten, Hardt, Cornelia, Lang, Philipp A. and Lang, Karl S. (2016). High Frequencies of Anti-Host Reactive CD8(+) T Cells Ignore Non-Hematopoietic Antigen after Bone Marrow Transplantation in a Murine Model. Cell. Physiol. Biochem., 38 (4). S. 1343 - 1354. BASEL: KARGER. ISSN 1421-9778

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Abstract

Background: Graft versus host disease (GvHD) occurs in 20% of cases with patients having an MHC I matched bone marrow transplantation (BMT). Mechanisms causing this disease remain to be studied. Methods: Here we used a CD8(+) T cell transgenic mouse line (P14/CD45.1(+)) and transgenic DEE mice bearing ubiquitously the glycoprotein 33-41 (GP33) antigen derived from the major lymphocytic choriomeningitis virus (LCMV) epitope to study mechanisms of tolerance in anti-host reactive CD8(+) T cells after BMT. Results: We found that anti-host reactive CD8(+) T cells (P14 T cells) were not negatively selected in the thymus and that they were present in wild type (WT) recipient mice as well as in DEE recipient mice. Anti host reactive CD8(+) T cells ignored the GP33 antigen expressed ubiquitously by host cells but they could be activated ex vivo via LCMV-infection. Lipopolysaccha rides (LPS) induced transient cell damage in DEE mice bearing anti-host reactive CD8(+) T cells after BMT, suggesting that induction of host inflammatory response could break antigen ignorance. Introducing the GP33 antigen into BM cells led to deletion of anti-host reactive CD8(+) T cells. Conclusion: We found that after BMT anti-host reactive CD8(+) T cells ignored host antigen in recipients and that they were only deleted when host antigen was present in hematopoietic cells. Moreover, [PS-induced immune activation contributed to induction of alloreactivity of anti-host reactive CD8(+) T cells after BMT. Copyright (C) 2016 S. Karger AG, Basel

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Gassa, AsmaeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kalkavan, HalimeUNSPECIFIEDorcid.org/0000-0002-0090-4080UNSPECIFIED
Jian, FuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Duhan, VikasUNSPECIFIEDorcid.org/0000-0002-6337-8225UNSPECIFIED
Khairnar, VishalUNSPECIFIEDorcid.org/0000-0002-3584-7530UNSPECIFIED
Shaabani, NamirUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Honke, NadineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Carpinteiro, AlexanderUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Botezatu, LacrarnioaraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Crivello, PietroUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dolff, SebastianUNSPECIFIEDorcid.org/0000-0001-5549-1706UNSPECIFIED
Ferencik, StanislavUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Haussinger, DieterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Khandanpour, CyrusUNSPECIFIEDorcid.org/0000-0003-4655-6269UNSPECIFIED
Fleischhauer, KatharinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Witzke, OliverUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wahlers, ThorstenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hardt, CorneliaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lang, Philipp A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lang, Karl S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-290024
DOI: 10.1159/000443078
Journal or Publication Title: Cell. Physiol. Biochem.
Volume: 38
Number: 4
Page Range: S. 1343 - 1354
Date: 2016
Publisher: KARGER
Place of Publication: BASEL
ISSN: 1421-9778
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ACUTE LYMPHOBLASTIC-LEUKEMIA; ACUTE MYELOID-LEUKEMIA; REDUCED-INTENSITY; IN-VIVO; DISEASE; GRAFT; CYTOMEGALOVIRUS; RECIPIENTS; DONORS; REPERTOIREMultiple languages
Cell Biology; PhysiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/29002

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