Universität zu Köln

Spitkovsky, Dimitry, Lemke, Karen, Foerster, Tobias, Roemer, Robert, Wiedemeier, Stefan, Hescheler, Juergen, Sachinidis, Agapios and Gastrock, Gunter (2016). Generation of Cardiomyocytes in Pipe-Based Microbioreactor Under Segmented Flow. Cell. Physiol. Biochem., 38 (5). S. 1883 - 1897. BASEL: KARGER. ISSN 1421-9778

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Abstract

Background/Aims: Embryonic stem (ES) cells have got a broad range differentiation potential. The differentiation is initiated via aggregation of non-differentiated ES cells into embryoid body (EB) capable of multi-lineage development. However experimental variables present in standard differentiation techniques lead to high EB heterogeneity, affecting development into the cells of desired lineage, and do not support the process automatization and scalability. Methods: Here we present a novel pipe based microbioreactor (PBM) setup based on segmented flow, designed for spatial maintenance of temperature, nutrition supply, gas supply and sterility. Results: We verified PBM feasibility for continuous process generating cardiac cells starting from single ES cell suspension followed by EB formation for up to 10 days. The ES cells used in the study were genetically modified for cardiac-specific EGFP expression allowing optical monitoring of cardiomyocytes while EBs remained within PBM for up to 10 days. Efficiency of cardiac cells formation within PBM was similar compared to a standard hanging drop based protocol. Conclusion: Our findings ensure further development of microfluidic bioreactor technology to enable robust cardiomyocytes production for needs of drug screening, tissue engineering and other applications. (C) 2016 The Author(s) Published by S. Karger AG, Basel

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Spitkovsky, Dimitry UNSPECIFIED UNSPECIFIED UNSPECIFIED Lemke, Karen UNSPECIFIED UNSPECIFIED UNSPECIFIED Foerster, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Roemer, Robert UNSPECIFIED UNSPECIFIED UNSPECIFIED Wiedemeier, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Sachinidis, Agapios UNSPECIFIED UNSPECIFIED UNSPECIFIED Gastrock, Gunter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-290033
DOI:	10.1159/000445550
Journal or Publication Title:	Cell. Physiol. Biochem.
Volume:	38
Number:	5
Page Range:	S. 1883 - 1897
Date:	2016
Publisher:	KARGER
Place of Publication:	BASEL
ISSN:	1421-9778
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL-DERIVED CARDIOMYOCYTES; EMBRYONIC STEM-CELLS; DIFFERENTIATION; CULTIVATION Multiple languages Cell Biology; Physiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/29003