Universität zu Köln

Wolf, Alexander, Beller, Katharina, Groemminger, Sebastian, Hofmann, Wera, Sachse, Matthias and Fassunke, Jana (2016). Purification of Circulating Cell-Free DNA from Plasma and Urine Using the Automated Large-Volume Extraction on the QIAsymphony (R) SP Instrument. S. 179 - 186. CHAM: SPRINGER INT PUBLISHING AG. ISSN 0065-2598

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Abstract

Increasing sample numbers for screening and diagnostics using circulating cell-free DNA (ccfDNA) as analyte demands an automated solution for ccfDNA extraction. The efficiency of a new, automated, large volume ccfDNA extraction method was evaluated against a manual reference method. The new kit for automated ccfDNA extraction on the QIAsymphony showed a comparable yield of total ccfDNA from healthy donors as well as a comparable recovery of circulating cancer and fetal DNA. In conclusion, a new kit for automated ccfDNA extraction was established successfully.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wolf, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Beller, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Groemminger, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Hofmann, Wera UNSPECIFIED UNSPECIFIED UNSPECIFIED Sachse, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Fassunke, Jana UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-292511
DOI:	10.1007/978-3-319-42044-8_33
Title of Book:	Advances in Experimental Medicine and Biology
Series Name:	Adv.Exp.Med.Biol.
Volume:	924
Page Range:	S. 179 - 186
Date:	2016
Publisher:	SPRINGER INT PUBLISHING AG
Place of Publication:	CHAM
ISSN:	0065-2598
ISBN:	978-3-319-42044-8; 978-3-319-42042-4
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Biochemistry & Molecular Biology; Medicine, Research & Experimental Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/29251