Universität zu Köln

Flegel, Caroline, Vogel, Felix, Hofreuter, Adrian, Schreiner, Benjamin S. P., Osthold, Sandra, Veitinger, Sophie, Becker, Christian, Brockmeyer, Norbert H., Muschol, Michael, Wennemuth, Gunther ORCID: 0000-0003-3313-2475, Altmueller, Janine, Hatt, Hanns and Gisselmann, Guenter (2016). Characterization of the Olfactory Receptors Expressed in Human Spermatozoa. Front. Mol. Biosci., 2. LAUSANNE: FRONTIERS MEDIA SA. ISSN 2296-889X

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Abstract

The detection of external cues is fundamental for human spermatozoa to locate the oocyte in the female reproductive tract. This task requires a specific chemoreceptor repertoire that is expressed on the surface of human spermatozoa, which is not fully identified to date. Olfactory receptors (ORs) are candidate molecules and have been attributed to be involved in sperm chemotaxis and chemokinesis, indicating an important role in mammalian spermatozoa. An increasing importance has been suggested for spermatozoal RNA, which led us to investigate the expression of all 387 OR genes. This study provides the first comprehensive analysis of OR transcripts in human spermatozoa of several individuals by RNA-Seq. We detected 91 different transcripts in the spermatozoa samples that could be aligned to annotated OR genes. Using stranded mRNA-Seq, we detected a class of these putative OR transcripts in an antisense orientation, indicating a different function, rather than coding for a functional OR protein. Nevertheless, we were able to detect OR proteins in various compartments of human spermatozoa, indicating distinct functions in human sperm. A panel of various OR ligands induced Ca2+ signals in human spermatozoa, which could be inhibited by mibefradil. This study indicates that a variety of ORs are expressed at the mRNA and protein level in human spermatozoa.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Flegel, Caroline UNSPECIFIED UNSPECIFIED UNSPECIFIED Vogel, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED Hofreuter, Adrian UNSPECIFIED UNSPECIFIED UNSPECIFIED Schreiner, Benjamin S. P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Osthold, Sandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Veitinger, Sophie UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Brockmeyer, Norbert H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Muschol, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Wennemuth, Gunther UNSPECIFIED orcid.org/0000-0003-3313-2475 UNSPECIFIED Altmueller, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED Hatt, Hanns UNSPECIFIED UNSPECIFIED UNSPECIFIED Gisselmann, Guenter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-292710
DOI:	10.3389/fmolb.2015.00073
Journal or Publication Title:	Front. Mol. Biosci.
Volume:	2
Date:	2016
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	2296-889X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CATSPER CHANNEL; RNA; GENES; CELLS; CA2+; IDENTIFICATION; SPECIFICITY; MEMBERS; BINDING; FAMILY Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/29271