Universität zu Köln

Ahmed, Mohammed, Sapp, Melanie ORCID: 0000-0002-0817-5425, Prior, Thomas, Karssen, Gerrit and Back, Matthew Alan (2016). Technological advancements and their importance for nematode identification. Soil, 2 (2). S. 257 - 271. GOTTINGEN: COPERNICUS GESELLSCHAFT MBH. ISSN 2199-398X

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Abstract

Nematodes represent a species-rich and morphologically diverse group of metazoans known to inhabit both aquatic and terrestrial environments. Their role as biological indicators and as key players in nutrient cycling has been well documented. Some plant-parasitic species are also known to cause significant losses to crop production. In spite of this, there still exists a huge gap in our knowledge of their diversity due to the enormity of time and expertise often involved in characterising species using phenotypic features. Molecular methodology provides useful means of complementing the limited number of reliable diagnostic characters available for morphology-based identification. We discuss herein some of the limitations of traditional taxonomy and how molecular methodologies, especially the use of high-throughput sequencing, have assisted in carrying out large-scale nematode community studies and characterisation of phytonematodes through rapid identification of multiple taxa. We also provide brief descriptions of some the current and almost-outdated high-throughput sequencing platforms and their applications in both plant nematology and soil ecology.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ahmed, Mohammed UNSPECIFIED UNSPECIFIED UNSPECIFIED Sapp, Melanie UNSPECIFIED orcid.org/0000-0002-0817-5425 UNSPECIFIED Prior, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Karssen, Gerrit UNSPECIFIED UNSPECIFIED UNSPECIFIED Back, Matthew Alan UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-293448
DOI:	10.5194/soil-2-257-2016
Journal or Publication Title:	Soil
Volume:	2
Number:	2
Page Range:	S. 257 - 271
Date:	2016
Publisher:	COPERNICUS GESELLSCHAFT MBH
Place of Publication:	GOTTINGEN
ISSN:	2199-398X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ROOT-KNOT NEMATODES; PLANT-PARASITIC NEMATODES; MELOIDOGYNE-CHITWOODI; MOLECULAR DIAGNOSTICS; M-FALLAX; PHYLOGENETIC-RELATIONSHIPS; BIOLOGICAL-CONTROL; ENZYME PHENOTYPES; RIBOSOMAL-RNA; DNA BARCODE Multiple languages Soil Science Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/29344