Universität zu Köln

Nissler, Robert, Bader, Oliver ORCID: 0000-0002-1534-440X, Dohmen, Maria, Walter, Sebastian G., Noll, Christine, Selvaggio, Gabriele, Gross, Uwe and Kruss, Sebastian (2020). Remote near infrared identification of pathogens with multiplexed nanosensors. Nat. Commun., 11 (1). BERLIN: NATURE RESEARCH. ISSN 2041-1723

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Abstract

Infectious diseases are worldwide a major cause of morbidity and mortality. Fast and specific detection of pathogens such as bacteria is needed to combat these diseases. Optimal methods would be non-invasive and without extensive sample-taking/processing. Here, we developed a set of near infrared (NIR) fluorescent nanosensors and used them for remote fingerprinting of clinically important bacteria. The nanosensors are based on single-walled carbon nanotubes (SWCNTs) that fluoresce in the NIR optical tissue transparency window, which offers ultra-low background and high tissue penetration. They are chemically tailored to detect released metabolites as well as specific virulence factors (lipopolysaccharides, siderophores, DNases, proteases) and integrated into functional hydrogel arrays with 9 different sensors. These hydrogels are exposed to clinical isolates of 6 important bacteria (Staphylococcus aureus, Escherichia coli,...) and remote (>= 25cm) NIR imaging allows to identify and distinguish bacteria. Sensors are also spectrally encoded (900nm, 1000nm, 1250nm) to differentiate the two major pathogens P. aeruginosa as well as S. aureus and penetrate tissue (>5mm). This type of multiplexing with NIR fluorescent nanosensors enables remote detection and differentiation of important pathogens and the potential for smart surfaces. Fast and specific detection of pathogenic bacteria is needed to combat infections. Here the authors generate an array of near-infrared biosensors based on carbon nanotubes to detect released metabolites and virulence factors and use them to distinguish pathogens such as S. aureus and P. aeruginosa.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Nissler, Robert UNSPECIFIED UNSPECIFIED UNSPECIFIED Bader, Oliver UNSPECIFIED orcid.org/0000-0002-1534-440X UNSPECIFIED Dohmen, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Walter, Sebastian G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Noll, Christine UNSPECIFIED UNSPECIFIED UNSPECIFIED Selvaggio, Gabriele UNSPECIFIED UNSPECIFIED UNSPECIFIED Gross, Uwe UNSPECIFIED UNSPECIFIED UNSPECIFIED Kruss, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-307400
DOI:	10.1038/s41467-020-19718-5
Journal or Publication Title:	Nat. Commun.
Volume:	11
Number:	1
Date:	2020
Publisher:	NATURE RESEARCH
Place of Publication:	BERLIN
ISSN:	2041-1723
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FLUORESCENT CARBON NANOTUBES; PSEUDOMONAS-FLUORESCENS; BACTERIA; SENSOR; IRON; NANOMATERIALS; SIDEROPHORES; RECOGNITION; SEPARATION; STABILITY Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/30740