Universität zu Köln

Bruno, Giuliana, Wenske, Sebastian, Lackmann, Jan-Wilm, Lalk, Michael ORCID: 0000-0002-9230-0267, von Woedtke, Thomas ORCID: 0000-0002-1097-4832 and Wende, Kristian ORCID: 0000-0001-5217-0683 (2020). On the Liquid Chemistry of the Reactive Nitrogen Species Peroxynitrite and Nitrogen Dioxide Generated by Physical Plasmas. Biomolecules, 10 (12). BASEL: MDPI. ISSN 2218-273X

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Abstract

Cold physical plasmas modulate cellular redox signaling processes, leading to the evolution of a number of clinical applications in recent years. They are a source of small reactive species, including reactive nitrogen species (RNS). Wound healing is a major application and, as its physiology involves RNS signaling, a correlation between clinical effectiveness and the activity of plasma-derived RNS seems evident. To investigate the type and reactivity of plasma-derived RNS in aqueous systems, a model with tyrosine as a tracer was utilized. By high-resolution mass spectrometry, 26 different tyrosine derivatives including the physiologic nitrotyrosine were identified. The product pattern was distinctive in terms of plasma parameters, especially gas phase composition. By scavenger experiments and isotopic labelling, gaseous nitric dioxide radicals and liquid phase peroxynitrite ions were determined as dominant RNS. The presence of water molecules in the active plasma favored the generation of peroxynitrite. A pilot study, identifying RNS driven post-translational modifications of proteins in healing human wounds after the treatment with cold plasma (kINPen), demonstrated the presence of in vitro determined chemical pathways. The plasma-driven nitration and nitrosylation of tyrosine allows the conclusion that covalent modification of biomolecules by RNS contributes to the clinically observed impact of cold plasmas.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Bruno, Giuliana UNSPECIFIED UNSPECIFIED UNSPECIFIED Wenske, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Lackmann, Jan-Wilm UNSPECIFIED UNSPECIFIED UNSPECIFIED Lalk, Michael UNSPECIFIED orcid.org/0000-0002-9230-0267 UNSPECIFIED von Woedtke, Thomas UNSPECIFIED orcid.org/0000-0002-1097-4832 UNSPECIFIED Wende, Kristian UNSPECIFIED orcid.org/0000-0001-5217-0683 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-309703
DOI:	10.3390/biom10121687
Journal or Publication Title:	Biomolecules
Volume:	10
Number:	12
Date:	2020
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2218-273X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROTEIN-TYROSINE NITRATION; ATMOSPHERIC-PRESSURE PLASMA; NITRIC-OXIDE; SINGLET OXYGEN; ERGOTHIONEINE; OXIDATION; ACID; HYDROXYLATION; GLUTATHIONE; SUPEROXIDE Multiple languages Biochemistry & Molecular Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/30970