Universität zu Köln

Mann, Marcus, Serif, Manuel ORCID: 0000-0002-0584-6748, Wrobel, Thomas, Eisenhut, Marion ORCID: 0000-0002-2743-8630, Madhuri, Shvaita ORCID: 0000-0002-1190-2507, Flachbart, Samantha, Weber, Andreas Pm, Lepetit, Bernard, Wilhelm, Christian and Kroth, Peter G. (2020). The Aureochrome Photoreceptor PtAUREO1a Is a Highly Effective Blue Light Switch in Diatoms. iScience, 23 (11). CAMBRIDGE: CELL PRESS. ISSN 2589-0042

Full text not available from this repository.

Abstract

Aureochromes represent a unique type of blue light photoreceptors that possess a blue light sensing flavin-binding LOV-domain and a DNA-binding bZIP domain, thus being light-driven transcription factors. The diatom Phaeodactylum tricornutum, a member of the essential marine primary producers, possesses four aureo-chromes (PtAUREO1a, 1b, 1c, 2). Here we show a dramatic change in the global gene expression pattern of P. tricornutum wild-type cells after a shift from red to blue light. About 75% of the genes show significantly changed transcript levels already after 10 and 60 min of blue light exposure, which includes genes of major transcription factors as well as other photoreceptors. Very surprisingly, this light-induced regulation of gene expression is almost completely inhibited in independent PtAureo1a knockout lines. Such a massive and fast transcriptional change depending on one single photoreceptor is so far unprecedented. We conclude that PtAUREO1a plays a key role in diatoms upon blue light exposure.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mann, Marcus UNSPECIFIED UNSPECIFIED UNSPECIFIED Serif, Manuel UNSPECIFIED orcid.org/0000-0002-0584-6748 UNSPECIFIED Wrobel, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Eisenhut, Marion UNSPECIFIED orcid.org/0000-0002-2743-8630 UNSPECIFIED Madhuri, Shvaita UNSPECIFIED orcid.org/0000-0002-1190-2507 UNSPECIFIED Flachbart, Samantha UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, Andreas Pm UNSPECIFIED UNSPECIFIED UNSPECIFIED Lepetit, Bernard UNSPECIFIED UNSPECIFIED UNSPECIFIED Wilhelm, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Kroth, Peter G. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-310967
DOI:	10.1016/j.isci.2020.101730
Journal or Publication Title:	iScience
Volume:	23
Number:	11
Date:	2020
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2589-0042
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PHAEODACTYLUM-TRICORNUTUM; MONOMERIC AUREOCHROME-1; INDUCED DIMERIZATION; CELL-DIVISION; LOV; CRYPTOCHROME; ACCLIMATION; EVOLUTION; RESPONSES; FAMILIES Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31096