Universität zu Köln

Osae, Eugene A., Bullock, Tiffany, Chintapalati, Madhavi, Brodesser, Susanne, Hanlon, Samuel, Redfern, Rachel, Steven, Philipp ORCID: 0000-0001-6892-3619, Smith, C. Wayne, Rumbaut, Rolando E. and Burns, Alan R. (2020). Obese Mice with Dyslipidemia Exhibit Meibomian Gland Hypertrophy and Alterations in Meibum Composition and Aqueous Tear Production. Int. J. Mol. Sci., 21 (22). BASEL: MDPI. ISSN 1422-0067

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Abstract

Background: Dyslipidemia may be linked to meibomian gland dysfunction (MGD) and altered meibum lipid composition. The purpose was to determine if plasma and meibum cholesteryl esters (CE), triglycerides (TG), ceramides (Cer) and sphingomyelins (SM) change in a mouse model of diet-induced obesity where mice develop dyslipidemia. Methods: Male C57/BL6 mice (8/group, age = 6 wks) were fed a normal (ND; 15% kcal fat) or an obesogenic high-fat diet (HFD; 42% kcal fat) for 10 wks. Tear production was measured and meibography was performed. Body and epididymal adipose tissue (eAT) weights were determined. Nano-ESI-MS/MS and LC-ESI-MS/MS were used to detect CE, TG, Cer and SM species. Data were analyzed by principal component analysis, Pearson's correlation and unpaired t-tests adjusted for multiple comparisons; significance set at p <= 0.05. Results: Compared to ND mice, HFD mice gained more weight and showed heavier eAT and dyslipidemia with higher levels of plasma CE, TG, Cer and SM. HFD mice had hypertrophic meibomian glands, increased levels of lipid species acylated by saturated fatty acids in plasma and meibum and excessive tear production. Conclusions: The majority of meibum lipid species with saturated fatty acids increased with HFD feeding with evidence of meibomian gland hypertrophy and excessive tearing. The dyslipidemia is associated with altered meibum composition, a key feature of MGD.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Osae, Eugene A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bullock, Tiffany UNSPECIFIED UNSPECIFIED UNSPECIFIED Chintapalati, Madhavi UNSPECIFIED UNSPECIFIED UNSPECIFIED Brodesser, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Hanlon, Samuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Redfern, Rachel UNSPECIFIED UNSPECIFIED UNSPECIFIED Steven, Philipp UNSPECIFIED orcid.org/0000-0001-6892-3619 UNSPECIFIED Smith, C. Wayne UNSPECIFIED UNSPECIFIED UNSPECIFIED Rumbaut, Rolando E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Burns, Alan R. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-312890
DOI:	10.3390/ijms21228772
Journal or Publication Title:	Int. J. Mol. Sci.
Volume:	21
Number:	22
Date:	2020
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	1422-0067
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FATTY-ACID-COMPOSITION; LIPID-COMPOSITION; METABOLIC COMPLICATIONS; INTERNATIONAL WORKSHOP; DYSFUNCTION REPORT; OCULAR SURFACE; MOUSE MODEL; HYPERLIPIDEMIA; FILM; SUBCOMMITTEE Multiple languages Biochemistry & Molecular Biology; Chemistry, Multidisciplinary Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31289